Mechanism of Proton Relaxation for Enzyme-Manipulated, Multicomponent Gold-Magnetic Nanoparticle Chains

被引：8

作者：

Jaganathan, Hamsa ^{[2
]}

Gieseck, Richard L. ^{[2
]}

Hudson, Katherine ^{[2
]}

Kellogg, Michael ^{[2
]}

Ramaswamy, Aneesh K. ^{[2
]}

Raver, Kimberly E. ^{[2
]}

Smith, Tyler ^{[2
]}

Vacchiano, Ashley N. ^{[2
]}

Wager, Andrew ^{[2
]}

Ivanisevic, Albena ^{[1
,2
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[2] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA

来源：

CHEMPHYSCHEM | 2010年 / 11卷 / 17期

关键词：

DNA; gold; MRI contrast agents; nanoparticles; NMR spectroscopy; MULTIFUNCTIONAL NANOPARTICLES; SHELL NANOPARTICLES; OXIDE; CORE; FABRICATION; DNA; CONTRAST; MRI; FE; ASSEMBLIES;

D O I：

10.1002/cphc.201000397

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Longitudinal and transverse relaxation times of multicomponent nanoparticle (NP) chains are investigated for their potential use as multifunctional imaging agents in magnetic resonance imaging (MRI). Gold NPs (ca. 5 nm) are arranged linearly along double-stranded DNA, creating gold NP chains. After cutting gold NP chains with restriction enzymes (EcoRI or BamHI), multicomponent NP chains are formed through a ligation reaction with enzyme-cut, superparamagnetic NP chains. We evaluate the changes in relaxation times for different constructs of gold-iron oxide NP chains and gold-cobalt iron oxide NP chains using 300 MHz H-1 NMR. In addition, the mechanism of proton relaxation for multicomponent NP chains is examined. The results indicate that relaxation times are dependent on the one-dimensional structure and the amount of superparamagnetic NP chains present in the multicomponent constructs. Multicomponent NP chains arranged on doublestranded DNA provide a feasible method for fabrication of multifunctional imaging agents that improve relaxation times effectively for MRI applications.

引用

页码：3664 / 3672

页数：9