Suppression of Intracellular Calcium Levels and Inhibition of Degranulation in RBL-2H3 Mast Cells by the Sesquiterpene Lactone Parthenolide

被引：5

作者：

Hong, JangJa ^{[1
,2
]}

Aoyama, Suzue ^{[3
]}

Hirasawa, Noriyasu ^{[3
]}

Zee, OkPyo ^{[2
]}

Ishihara, Kenji ^{[4
]}

Hashida, Chika

Kimura, Michio

Seyama, Toshio

Ohuchi, Kazuo

机构：

[1] Yasuda Womens Univ, Lab Life Sci, Fac Pharm, Asaminami Ku, Hiroshima 7310153, Japan

[2] Sungkyunkwan Univ, Lab Pharmacognosy, Grad Sch Pharm, Suwon, Kyungi Do, South Korea

[3] Tohoku Univ, Lab Pharmacotherapy Life Style Related Dis, Grad Sch Pharmaceut Sci, Sendai, Miyagi 980, Japan

[4] Ibaraki Univ, Lab Med Sci, Fac Educ, Mito, Ibaraki, Japan

来源：

PLANTA MEDICA | 2011年 / 77卷 / 03期

关键词：

Tanacetum parthenium (L.) Sch Bip; Asteraceae; parthenolide; RBL-2H3 mast cells; degranulation; beta-hexosaminidase; intracellular calcium; FACTOR-KAPPA-B; TANACETUM-VULGARE; KINASE COMPLEX; EXTRACT; RATS;

D O I：

10.1055/s-0030-1250221

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Pretreatment with parthenolide for 60 min inhibited the antigen-induced degranulation of RBL-2H3 mast cells; the IC(50) value being 4.5 +/- 0.4 mu M. The inhibition was not due to suppression of the phosphatidylinositol 3-kinase pathway because the antigen-induced phosphorylation of Akt was not inhibited by parthenolide. The antigen-induced increase in intracellular calcium levels was prevented by parthenolide, suggesting that parthenolide inhibited the antigen-induced degranulation by suppressing an increase in intracellular calcium levels. In support of this, parthenolide was found to prevent ionomycin-induced degranulation by inhibiting an increase in intracellular calcium levels. Therefore, parthenolide inhibits the degranulation of mast cells by preventing an increase in intracellular calcium levels.

引用

页码：252 / U108

页数：5

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