The hydrolytic efficiency and synergistic action of recombinant xylan-degrading enzymes on xylan isolated from sugarcane bagasse

被引：22

作者：

Li, Hailong ^{[1
,3
]}

Wu, Hongli ^{[2
]}

Xiong, Lian ^{[1
,3
]}

Chen, Xuefang ^{[1
,3
]}

Wang, Can ^{[1
,3
]}

Qi, Gaoxiang ^{[1
,4
]}

Huang, Chao ^{[1
,3
]}

Guo, Haijun ^{[1
,3
]}

Luo, Mutan ^{[1
,4
]}

Liu, Jian ^{[2
]}

Long, Minnan ^{[2
]}

Chen, Xinde ^{[1
,3
]}

机构：

[1] Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Laboratoryof New & Renewable E, Guangzhou 510640, Guangdong, Peoples R China

[2] Xiamen Univ, Coll Energy, Xiamen 361102, Peoples R China

[3] Chinese Acad Sci, Guangzhou Inst Energy Convers, R&D Ctr Xuyi Attapulgite Appl Technol, Xuyi 21170, Peoples R China

[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2017年 / 175卷

基金：

中国国家自然科学基金;

关键词：

Hydrolytic efficiency; Synergistic action; Sugarcane bagasse; Xylan; Xylan-degrading enzymes; GRADED ETHANOL PRECIPITATION; ALPHA-L-ARABINOFURANOSIDASES; ENZYMATIC-HYDROLYSIS; EUCALYPTUS-GRANDIS; HEMICELLULOSES; XYLOOLIGOSACCHARIDES; OLIGOSACCHARIDES; FAMILIES; MONOSACCHARIDES; ARABINOXYLANS;

D O I：

10.1016/j.carbpol.2017.07.075

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Understanding the interaction mechanisms between xylan and xylan-degrading enzymes is beneficial to the efficient hydrolysis of xylan. Xylan from sugarcane bagasse (SB) was extracted and characterized. The effects of heat treatment and removal of side chains of SB xylan on the hydrolytic efficiency and synergistic action of endo-beta-1,4-xylanases (HoXyn1 1A and AnXyn10C), beta-xylosidases (AnXln3D), and alpha-L-arabinofuranosidases (AnAxh62A) were investigated. Results indicated that heat treatment of xylan can improve the hydrolytic efficiency of xylan-degrading enzymes, and it is essential for the efficient hydrolysis of xylan by HoXyn1 1A. The removal of arabinofuranosyl side chains of xylan by AnAxh62A before enzymatic hydrolysis reduced the hydrolytic efficiency of HoXyn1 1A and AnXyn10C on xylan. AnXyn10C was more efficient than HoXyn1 1A in hydrolysis of xylan, whereas HoXyn1 1A showed better synergistic action than AnXyn10C with AnAxh62A and AnXln3D in the hydrolysis of xylan. This study provides new insights on the enzymatic hydrolysis of SB into monosaccharides and xylo-oligosaccharides. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：199 / 206

页数：8

共 32 条

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