Rice ubiquitin promoters: deletion analysis and potential usefulness in plant transformation systems

被引：58

作者：

Wang, J ^{[1
]}

Oard, JH ^{[1
]}

机构：

[1] Louisiana State Univ, LSU Agr Ctr, Louisiana Agr Expt Stn, AgCtr Biotechnol Lab,Dept Agron, Baton Rouge, LA 70803 USA

来源：

PLANT CELL REPORTS | 2003年 / 22卷 / 02期

关键词：

gene silencing; promoter; Oryza sativa; transgenic plant; ubiquitin;

D O I：

10.1007/s00299-003-0657-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Strong constitutive promoters form a cornerstone for basic and applied research using transgenic plants. GUS (beta-glucuronidase) expression levels from constructs containing RUBQ1 or RUB2 rice ubiquitin promoters were 8- to 35-fold higher in transgenic rice [Oryza sativa (L.)] plants, respectively, when compared to the 35S promoter. Deletion analysis of the 5'-upstream region of RUBQ2 revealed a putative enhancer region that produced a 2.4-fold increase in transient GUS expression. Southern blot analysis showed that three to seven copies of the GUS gene were stably inserted into R0 and R1 plants and inherited in a monogenic fashion.

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收藏

页码：129 / 134

页数：6

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