Study on the Binding Equilibrium Between Surfactant FC-134 and DNA by Resonance Light Scattering Technique

被引：2

作者：

Wang, Ying Xi ^{[1
]}

Li, Ling ^{[1
]}

Xu, Zu Shun ^{[1
]}

Pan, Quan ^{[1
]}

Song, Gong Wu ^{[1
]}

机构：

[1] Hubei Univ, Minist Educ, Key Lab Synth & Applicat Organ Funct Mol, Wuhan 430062, Hubei Province, Peoples R China

来源：

JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY | 2011年 / 32卷 / 01期

关键词：

Binding equilibrium; DNA; fluoro surfactant; resonance light scattering; HSA SERUM ALBUMINS; NUCLEIC-ACIDS; IONIC SURFACTANTS; BOVINE BSA; SPECTROSCOPY; COMPLEX;

D O I：

10.1080/01932690903543014

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This article presents the study of binding equilibrium of fluoro surfactant FC-134 to DNA was studied by resonance light scattering. It was found that the interaction resulted in strongly enhanced resonance light scattering signals characterized by the RLS peak at 370nm. The binding constant Kf and the number of binding n were calculated according to the equilibrium equation, which was established based on the data of the resonance light scattering measurements at 370nm. It was found that the binding Kf and the number of binding n were 5.98x105 and 1.4, respectively.

引用

页码：114 / 119

页数：6

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