The Occurrence of Antibiotic Resistance Genes in an Urban River in Nepal

被引：19

作者：

Thakali, Ocean ^{[1
]}

Tandukar, Sarmila ^{[2
]}

Brooks, John P. ^{[3
]}

Sherchan, Samendra P. ^{[4
]}

Sherchand, Jeevan B. ^{[5
]}

Haramoto, Eiji ^{[2
]}

机构：

[1] Univ Yamanashi, Environm & Social Syst Sci Course, 4-3-11 Takeda, Kofu, Yamanashi 4008511, Japan

[2] Univ Yamanashi, Interdisciplinary Ctr River Basin Environm, 4-3-11 Takeda, Kofu, Yamanashi 4008511, Japan

[3] USDA ARS, Genet & Sustainable Agr Unit, 810 Highway 12 East, Mississippi State, MS 39762 USA

[4] Tulane Univ, Dept Global Environm Hlth Sci, 1440 Canal St,Suite 2100, New Orleans, LA 70112 USA

[5] Tribhuvan Univ, Inst Med, Kathmandu 1524, Nepal

来源：

WATER | 2020年 / 12卷 / 02期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

antibiotic resistance gene; integron; quantitative PCR; river water; REAL-TIME PCR; WASTE-WATER; KATHMANDU VALLEY; BACTERIAL LOAD; QUANTIFICATION;

D O I：

10.3390/w12020450

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Urban rivers affected by anthropogenic activities can act as reservoirs of antibiotic resistance genes (ARGs). This study aimed to describe the occurrence of selected ARGs (bla(TEM), ermF, mecA, and tetA) and a class 1 integron (Mtn) in an urban river in Nepal. A total of 18 water samples were collected periodically from upstream, midstream, and downstream sites along the Bagmati River over a 1-year period. All ARGs except mecA and intIl were consistently detected by a quantitative polymerase chain reaction in the midstream and downstream sites, with concentrations ranging from 3.1 to 7.8 log copies/mL. ARG abundance was significantly lower at the upstream site (p < 0.05), reflecting the impact of anthropogenic activities on increasing concentrations of ARGs at midstream and downstream sites. Our findings demonstrate the presence of clinically relevant ARGs in the urban river water of Nepal, suggesting a need for mitigating strategies to prevent the spread of antibiotic resistance in the environment.

引用

页数：7

共 32 条

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