In vitro anti-rotavirus activity of polyphenol compounds isolated from the roots of Glycyrrhiza uralensis

被引:79
作者
Kwon, Hyung-Jun [1 ,2 ]
Kim, Ha-Hyun [1 ,2 ]
Ryu, Young Bae [1 ,2 ]
Kim, Jang Hoon [1 ,2 ]
Jeong, Hyung Jae [1 ,2 ]
Lee, Seung-Woong [1 ,2 ]
Chang, Jong Sun [1 ,2 ]
Cho, Kyoung-Oh [3 ]
Rho, Mun-Chual [1 ,2 ]
Park, Su-Jin [1 ,2 ]
Lee, Woo Song [1 ,2 ]
机构
[1] Korea Res Inst Biosci & Biotechnol, Ecofriendly Biomat Res Ctr, Jeongeup 580185, South Korea
[2] Korea Res Inst Biosci & Biotechnol, AI Control Mat Res Ctr, Jeongeup 580185, South Korea
[3] Chonnam Natl Univ, Coll Vet Med, Biotherapy Human Resources Ctr, Kwangju 500757, South Korea
关键词
Glycyrrhiza uralensis; Anti-rotavirus; Viral replication; Viral absorption; Hemagglutinin; ANTIVIRAL ACTIVITY; ROTAVIRUS; INHIBITION; VIRUS; COMPONENT; CULTURES; EXTRACT;
D O I
10.1016/j.bmc.2010.07.073
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We evaluated the ability of six polyphenols isolated from the roots of Glycyrrhiza uralensis to inactivate rotaviruses, specially G5P[7] and G8P[7]. Upon finding that all polyphenols possessed anti-rotavirus activity, we evaluated whether these properties were attributable to direct inhibition of the binding of rotavirus to cells and/or to inhibition of viral replication. Using the virucidal assay, we found that all six compounds directly inhibited rotavirus binding, with activity being dependent on the type of virus. The 50% effective inhibitory concentrations (EC50) of the six compounds were 18.7-69.5 mu M against G5P[7] and 14.7-88.1 mu M against G8P[7], respectively. Five of the six compounds inhibited hemagglutination activity. Moreover, the CPE inhibition assay showed that five compounds inhibited viral replication with EC50 values of 12.1-24.0 mu M against G5P[7] and 12.0-42.0 mu M against G8P[7], respectively. RT-PCR showed that the compounds suppressed viral RNA synthesis in TF-104 cells. Interestingly, the anti-rotavirus activities of four compounds were attributable to inhibition of both viral absorption and viral replication. These results suggest that compounds isolated from the roots of G. uralensis may be potent anti-rotavirus agents in vivo, acting by inhibiting both viral absorption and viral replication. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:7668 / 7674
页数:7
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