Labeling during cleavage of nucleic acids for their detection on DNA chips

被引：0

作者：

Laayoun, A

Sothier, I

Menou, L

Bernal-Méndez, E

Kotera, M

Bourget, C

Trévisiol, E

Lhomme, J

Troesch, A

机构：

[1] BioMerieux SA Adv Technol, Adv Technol, F-69280 Marcy Letoile, France

[2] Univ Grenoble 1, CNRS, UMR5616,LEDSS, Grenoble, France

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2003年 / 22卷 / 5-8期

关键词：

DNA chip; labeling; cleavage; aryldiazomethane;

D O I：

10.1081/NCN-120022603

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new and efficient strategy for labeling of nuleic acids prior to their hybridization on high density DNA chip has been developed. Our approach which combines the fragmentation and the labeling is based on the reactivity of the terminal phosphates of cleaved DNA and RNA fragments with a reporter molecule bearing aryldiazomethane group.

引用

页码：649 / 652

页数：4

共 7 条

[1] Metal ion-catalyzed nucleic acid alkylation and fragmentation [J].