Mouse tail skin wholemount staining and intravital calcium imaging

被引：2

作者：

Xie, Yuhua ^{[1
]}

Chen, Ting ^{[1
,2
]}

机构：

[1] Natl Inst Biol Sci, Beijing, Peoples R China

[2] Tsinghua Univ, Tsinghua Inst Multidisciplinary Biomed Res, Beijing, Peoples R China

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 01期

关键词：

Developmental biology; Microscopy; Model Organisms; Stem Cells;

D O I：

10.1016/j.xpro.2022.101235

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mouse tail skin offers multiple benefits for skin research. Here, we present a protocol detailing high resolution tail skin whole mount staining and in vivo calcium imaging of hair follicle stem cells (HFSCs) using Sox9creERT2, GCaMP6s, and Ai14 mice. This approach enables the study of hair follicles and stem cells in different physiological and pathological conditions. The applications of the protocol include visualization of calcium signaling in other cell types with Cre/CreER lines or analyzing other cellular features with different reporter lines. For complete details on the use and execution of this protocol, please refer to Xie et al. (2022).

引用

页数：12

共 30 条

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