Molecular and functional characterisation of a mannose-binding lectin-like gene from Japanese sea bass (Lateolabrax japonicus)

被引：4
作者：
Dang, Yunfei [1 ,2 ]
Nie, Li [1 ,2 ]
Chen, Jiong [1 ,2 ,3 ]
机构：
[1] Ningbo Univ, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China
[2] Ningbo Univ, Sch Marine Sci, Lab Biochem & Mol Biol, Ningbo 315832, Peoples R China
[3] Ningbo Univ, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315832, Peoples R China
来源：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2020年 / 104卷
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
Bacterial agglutination; GalBL; Japanese sea bass; Sugar binding; Survival rate; C-TYPE LECTIN; CARBOHYDRATE-RECOGNITION DOMAIN; CHANNEL CATFISH; IMMUNE-RESPONSE; IDENTIFICATION; COMPLEMENT; FISH; EXPRESSION; BACTERIA; PEPTIDOGLYCAN;
D O I：
10.1016/j.dci.2019.103558
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Mannose-binding lectin (MBL) plays an important role in host immune responses against pathogens. LjMBL-like-1 was identified from Japanese sea bass (Lateolabrax japonicas), which has selectivity for galactose. Herein, this lectin might be better designated as galactose-binding lectin (LjGalBL-1). LjGalBL-1 transcripts were detected in all tested tissues, with highest expression in liver. Upon Vibrio harveyi infection, LjGalBL-1 mRNA expression was increased in major immune-related tissues, and protein levels in serum were also upregulated. Recombinant. LjGalBL-1 (rLjGalBL-1) bound to monosaccharides and polysaccharides, and both rLjGalBL-1 and native LjGalBL1 (nLjGaIBL-1) agglutinated three Gram-positive bacteria (Staphylococcus aureus, Streptococcus iniae and Micrococcus luteus) and four Gram-negative bacteria (Aeromonas hydrophila, Edwardsiella tarda, Vibrio anguillarum and V. harveyi) in a Ca2+-dependent manner in vitro. Moreover, rLjGalBL-1 increased the survival rate of V. harveyi-infected fish and decreased bacterial load in liver, spleen, kidney and blood. Thus, LjGalBL-1 protects L. japonicas against V. harveyi infection.
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页数：10
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