Phytophthora infection signals-induced translocation of NAC089 is required for endoplasmic reticulum stress response-mediated plant immunity

被引:17
作者
Ai, Gan [1 ]
Zhu, Hai [1 ]
Fu, Xiaowei [1 ]
Liu, Jin [1 ]
Li, Tianli [1 ]
Cheng, Yang [1 ]
Zhou, Yang [1 ]
Yang, Kun [1 ]
Pan, Weiye [1 ]
Zhang, Huanxin [1 ]
Wu, Zishan [1 ]
Dong, Saiyu [1 ]
Xia, Yeqiang [1 ]
Wang, Yuanchao [1 ]
Xia, Ai [1 ]
Wang, Yiming [1 ]
Dou, Daolong [1 ]
Jing, Maofeng [1 ]
机构
[1] Nanjing Agr Univ, Coll Plant Protect, Key Lab Plant Immun, Nanjing 210095, Peoples R China
基金
中国国家自然科学基金;
关键词
PAMPs; plant immunity; ER stress; programed cell death; Phytophthora; plant resistance; UNFOLDED PROTEIN RESPONSE; TRANSCRIPTION FACTOR; QUALITY-CONTROL; ARABIDOPSIS; EFFECTORS; DEATH; RESISTANCE; PATHOGENS; ENTRY;
D O I
10.1111/tpj.15425
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plants deploy various immune receptors to recognize pathogen-derived extracellular signals and subsequently activate the downstream defense response. Recently, increasing evidence indicates that the endoplasmic reticulum (ER) plays a part in the plant defense response, known as ER stress-mediated immunity (ERSI), that halts pathogen infection. However, the mechanism for the ER stress response to signals of pathogen infection remains unclear. Here, we characterized the ER stress response regulator NAC089, which was previously reported to positively regulate programed cell death (PCD), functioning as an ERSI regulator. NAC089 translocated from the ER to the nucleus via the Golgi in response to Phytophthora capsici culture filtrate (CF), which is a mixture of pathogen-associated molecular patterns (PAMPs). Plasma membrane localized co-receptor BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1) was required for the CF-mediated translocation of NAC089. The nuclear localization of NAC089, determined by the NAC domain, was essential for immune activation and PCD. Furthermore, NAC089 positively contributed to host resistance against the oomycete pathogen P. capsici and the bacteria pathogen Pseudomonas syringae pv. tomato (Pst) DC3000. We also proved that NAC089-mediated immunity is conserved in Nicotiana benthamiana. Together, we found that PAMP signaling induces the activation of ER stress in plants, and that NAC089 is required for ERSI and plant resistance against pathogens.
引用
收藏
页码:67 / 80
页数:14
相关论文
共 49 条
[31]   Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants [J].
Nuruzzaman, Mohammed ;
Sharoni, Akhter M. ;
Kikuchi, Shoshi .
FRONTIERS IN MICROBIOLOGY, 2013, 4
[32]   Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process [J].
Petre, Benjamin ;
Contreras, Mauricio P. ;
Bozkurt, Tolga O. ;
Schattat, Martin H. ;
Sklenar, Jan ;
Schornack, Sebastian ;
Abd-El-Haliem, Ahmed ;
Castells-Graells, Roger ;
Lozano-Duran, Rosa ;
Dagdas, Yasin F. ;
Menke, Frank L. H. ;
Jones, Alexandra M. E. ;
Vossen, Jack H. ;
Robatzek, Silke ;
Kamoun, Sophien ;
Win, Joe .
PLANT CELL, 2021, 33 (05) :1447-1471
[33]   How Do Filamentous Pathogens Deliver Effector Proteins into Plant Cells? [J].
Petre, Benjamin ;
Kamoun, Sophien .
PLOS BIOLOGY, 2014, 12 (02)
[34]   Susceptibility factor RTP1 negatively regulates Phytophthora parasitica resistance via modulating UPR regulators bZIP60 and bZIP28 [J].
Qiang, Xiaoyu ;
Liu, Xingshao ;
Wang, Xiaoxue ;
Zheng, Qing ;
Kang, Lijuan ;
Gao, Xianxian ;
Wei, Yushu ;
Wu, Wenjie ;
Zhao, Hong ;
Shan, Weixing .
PLANT PHYSIOLOGY, 2021, 186 (02) :1269-1287
[35]   The Mutualistic Fungus Piriformospora indica Colonizes Arabidopsis Roots by Inducing an Endoplasmic Reticulum Stress-Triggered Caspase-Dependent Cell Death [J].
Qiang, Xiaoyu ;
Zechmann, Bernd ;
Reitz, Marco U. ;
Kogel, Karl-Heinz ;
Schaefer, Patrick .
PLANT CELL, 2012, 24 (02) :794-809
[36]   Arabidopsis transcription factors:: Genome-wide comparative analysis among eukaryotes [J].
Riechmann, JL ;
Heard, J ;
Martin, G ;
Reuber, L ;
Jiang, CZ ;
Keddie, J ;
Adam, L ;
Pineda, O ;
Ratcliffe, OJ ;
Samaha, RR ;
Creelman, R ;
Pilgrim, M ;
Broun, P ;
Zhang, JZ ;
Ghandehari, D ;
Sherman, BK ;
Yu, CL .
SCIENCE, 2000, 290 (5499) :2105-2110
[37]   Death by proteases in plants: whodunit [J].
Rotari, VI ;
He, R ;
Gallois, P .
PHYSIOLOGIA PLANTARUM, 2005, 123 (04) :376-385
[38]   Receptor quality control in the endoplasmic reticulum for plant innate immunity [J].
Saijo, Yusuke ;
Tintor, Nico ;
Lu, Xunli ;
Rauf, Philipp ;
Pajerowska-Mukhtar, Karolina ;
Haeweker, Heidrun ;
Dong, Xinnian ;
Robatzek, Silke ;
Schulze-Lefert, Paul .
EMBO JOURNAL, 2009, 28 (21) :3439-3449
[39]   A rapid and non-destructive screenable marker, FAST, for identifying transformed seeds of Arabidopsis thaliana [J].
Shimada, Takashi L. ;
Shimada, Tomoo ;
Hara-Nishimura, Ikuko .
PLANT JOURNAL, 2010, 61 (03) :519-528
[40]   NtbZIP60, an endoplasmic reticulum-localized transcription factor, plays a role in the defense response against bacterial pathogens in Nicotiana tabacum [J].
Tateda, Chika ;
Ozaki, Rei ;
Onodera, Yu ;
Takahashi, Yoshihiro ;
Yamaguchi, Koji ;
Berberich, Thomas ;
Koizumi, Nozomu ;
Kusano, Tomonobu .
JOURNAL OF PLANT RESEARCH, 2008, 121 (06) :603-611