Global comparison of gene expression profiles between intramuscular and subcutaneous adipocytes of neonatal landrace pig using microarray

被引：69

作者：

Zhou, Guixuan ^{[1
]}

Wang, Songbo ^{[1
]}

Wang, Zhonggang ^{[1
]}

Zhu, Xiaotong ^{[1
]}

Shu, Gang ^{[1
]}

Liao, Weiyi ^{[1
]}

Yu, Kaifan ^{[1
]}

Gao, Ping ^{[1
]}

Xi, Qianyun ^{[1
]}

Wang, Xiuqi ^{[1
]}

Zhang, Yongliang ^{[1
]}

Yuan, Li ^{[2
]}

Jiang, Qingyan ^{[1
]}

机构：

[1] S China Agr Univ, Coll Anim Sci, Lab Anim Physiol & Biochem, Guangzhou 510642, Guangdong, Peoples R China

[2] Xiamen Univ, Sch Life Sci, Key Lab, Minist Educ Cell Biol & Tumor Cell Engn, Xiamen 361005, Peoples R China

来源：

MEAT SCIENCE | 2010年 / 86卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Gene expression; Intramuscular adipocytes; Subcutaneous adipocytes; Cell differentiation; Microarray; ACID-BINDING PROTEINS; CONJUGATED LINOLEIC-ACID; BONE MORPHOGENETIC PROTEIN-4; STROMAL-VASCULAR CELLS; ADIPOSE-TISSUE; SKELETAL-MUSCLE; FAT-CONTENT; REGIONAL DIFFERENCES; DIFFERENTIATION; ADIPOGENESIS;

D O I：

10.1016/j.meatsci.2010.05.031

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The objective of this study was to compare the differences of gene expression profiles between intramuscular and subcutaneous adipocytes originated from the isolated preadipocytes in vitro. Cytosolic triglyceride determination indicated that subcutaneous adipocytes accumulated more lipid than intramuscular adipocytes did at the late stage of differentiation. Microarray assay revealed that 172 probes representing 133 genes were differentially expressed, among which 46 genes were highly expressed in intramuscular adipocytes and the other 87 genes were highly expressed in subcutaneous adipocytes. Real-time PCR confirmed that genes related to lipid metabolism, such as LPL, FABP4, FABP5 and OSBPL10, were predominantly expressed in subcutaneous adipocytes, whereas BMP4 and BMP7 were highly expressed in intramuscular adipocytes. The results indicated that the accumulation of lipid mass in subcutaneous adipocytes might be due to the highly expressed genes related to lipid metabolism, and the high levels of BMP4 and BMP7 in intramuscular adipocytes suggested that BMPs might be involved in the differentiation of intramuscular adipocytes. (C) 2010 The American Meat Science Association. Published by Elsevier Ltd. All rights reserved.

引用

页码：440 / 450

页数：11

共 53 条

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