Study on the interaction of Pb2+ and bovine serum albumin by fluorescence

被引：0
作者：
Wu, GH [1 ]
Wang, CH
机构：
[1] Anqing Teachers Coll, Dept Chem, Anqing 246003, Peoples R China
[2] Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China
来源：
SPECTROSCOPY AND SPECTRAL ANALYSIS | 2005年 / 25卷 / 02期
关键词：
BSA; fluorescence spectrum; Pb2+;
D O I：
暂无
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
The interaction of Pb2+ and bovine serum albumin(BSA) was studied under conditions similar to those in human bodies by fluorescence spectra. The results indicated that tryptophan and tyrosine, which were located in BSA, had a max fluorescence emission peak at 341 nm with an excitation wavelength of 283 mn. It was shown that Pb2+ had a powerful ability to quench the BSA fluorescence with a mechanism of a static process rather than a dynamic one. The apparent quenching constant K, was obtained to be 9.5 X 10(12) L-mol(-1)-s(-1) by Stern-Volmer equation. The apparent complexation constant of Pb-2-BSA is 1gK = 11.61. The nitrogen in BSA could coordinate with lead in Pb-2-BSA.
引用
收藏
页码：246 / 248
页数：3
相关论文
共 11 条
[1]  
Chi YH, 1999, CHEM J CHINESE U, V20, P1697
[2]  
FENG FJ, 2002, SICHUAN ENV, V21
[3]   BINDING OF CADMIUM(II) AND ZINC(II) TO HUMAN AND DOG SERUM ALBUMINS - AN EQUILIBRIUM DIALYSIS AND CD-113-NMR STUDY [J].
GOUMAKOS, W ;
LAUSSAC, JP ;
SARKAR, B .
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1991, 69 (12) :809-820
[4]  
GUO YJ, 1983, FLUORESCENCE EXPT TE
[5]  
Li S.T., 1998, J GUANGXI NORMAL U, V16, P43
[6]  
Li XJ, 1999, CHEM J CHINESE U, V20, P127
[7]  
MASUOKA J, 1993, J BIOL CHEM, V268, P21533
[8]  
TU CQ, 1996, GUANGXI SCI, V6, P279
[9]  
YANG BS, 1989, PROG BIOCHEM BIOPHYS, V16, P351
[10]  
Zha DM, 1999, SPECTROSC SPECT ANAL, V19, P788
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