Replacement of antibodies with bacteriophages in lateral flow assay of Salmonella Enteritidis

被引：50

作者：

Ilhan, Hasan ^{[1
]}

Tayyarcan, Emine Kubra ^{[2
]}

Caglayan, Mehmet Gokhan ^{[3
]}

Boyaci, Ismail Hakki ^{[2
]}

Saglam, Necdet ^{[4
]}

Tamer, Ugur ^{[5
]}

机构：

[1] Ordu Univ, Dept Chem, Fac Sci, TR-52200 Altinordu, Ordu, Turkey

[2] Hacettepe Univ, Dept Food Engn, Fac Engn, TR-06800 Ankara, Turkey

[3] Ankara Univ, Dept Analyt Chem, Fac Pharm, TR-06560 Ankara, Turkey

[4] Hacettepe Univ, Dept Nanotechnol, Fac Sci, TR-06800 Ankara, Turkey

[5] Gazi Univ, Dept Analyt Chem, Fac Pharm, TR-06330 Ankara, Turkey

来源：

BIOSENSORS & BIOELECTRONICS | 2021年 / 189卷

关键词：

Salmonella enteritidis; Bacteriophage; Lateral flow assay; Surface enhanced Raman scattering; RAPID DETECTION; IMMUNOASSAY; NANOPARTICLES; SENSITIVITY; BACTERIA;

D O I：

10.1016/j.bios.2021.113383

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this study, the analytical performance of bacteriophages for Salmonella Enteritidis was investigated using lateral flow assay (LFA) technique. The analytical performance characteristics of bacteriophages were compared with antibodies which are regularly used as analyte-specific agents in the lateral flow immunoassay test strip. Bacteriophages could be an alternative analyte-specific agents to antibodies in lateral flow assay testing of bacteria since they offer comparable sensitivity, specificity, and accuracy. In the present study, Surface Enhanced Raman Spectroscopy (SERS) and colorimetric measurements were combined in one platform and sensitive quantitation of target bacteria was accomplished with a total quantitative analysis time of less than 30 min. The developed Salmonella Enteritidis F5-4 phage-based LFA specifically responds to Salmonella Enteritidis, while lower SERS responses to different bacteria types including Bacillus subtilis, Micrococcus luteus, Escherichia coli, Salmonella Typhimurium were observed. The developed test strips were also applied for the determination of Salmonella Enteritidis in spiked chicken and egg samples.

引用

页数：8

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