A hybrid nanosensor based on novel fluorescent iron oxide nanoparticles for highly selective determination of Hg2+ ions in environmental samples

Iron oxide nanoparticle-based chemosensors composed of fluorescent components have recently been examined as promising platforms for the separation, imaging, and, especially, determination of heavy metal ions. Among the heavy metal ions, Hg2+ has the most toxic effects and poses an important risk to the environment and living organisms due to its prevalence, widespread use, and tendency to accumulate. In response to the immediate necessity for the sensitive and selective determination of Hg2+ ions, novel fluorescent iron oxide nanoparticles (Py@Fe2O3) were systematically prepared via surface modification with pyrene base fluoroionophore groups via a click reaction. The novel Py@Fe2O3 and fluoroionophore were characterized structurally, morphologically and thermally using MALDI-TOF, H-1, C-13 NMR, UV-Vis, FTIR, SEM, TEM and TGA. The effects of the pH, buffer concentration, initial concentration of the sensor, photostability and measurement time on the relative fluorescence signal of Py@Fe2O3 were evaluated and optimized for "turn-off'' determination of Hg2+. The LOD and LOQ were determined to be 3.650 nmol L-1 and 10.960 nmol L-1 in the linear working range of 0.010-1.000 mmol L-1 Hg2+. These values were lower than the permissible limit for Hg2+ in drinking water set by the World Health Organization. ICP-MS and a spike/recovery test were carried out to evaluate the sensor accuracy, and the results demonstrated that the "turn-off'' signal change of Py@Fe2O3 could be sensitively, easily, and selectively used for reliable quantification of Hg2+ in environmental samples.