Core/Shell Nanofibers with Embedded Liposomes as a Drug Delivery System

被引：187

作者：

Mickova, Andrea ^{[1
,2
]}

Buzgo, Matej ^{[1
,2
]}

Benada, Oldrich ^{[3
]}

Rampichova, Michala ^{[1
,2
]}

Fisar, Zdenek ^{[4
]}

Filova, Eva ^{[2
,5
]}

Tesarova, Martina ^{[6
]}

Lukas, David ^{[7
]}

Amler, Evzen ^{[1
,2
,5
]}

机构：

[1] Charles Univ Prague, Fac Med 2, Dept Biophys, Prague 15006 5, Czech Republic

[2] Acad Sci Czech Republ, Inst Expt Med, Vvi, Prague 14220 4, Czech Republic

[3] Acad Sci Czech Republ, Inst Microbiol, Vvi, Lab Mol Struct Characterizat, CR-14220 Prague 4, Czech Republic

[4] Charles Univ Prague, Fac Med 1, Dept Psychiat, Prague 15006 5, Czech Republic

[5] Czech Tech Univ, Fac Biomed Engn, Kladno 27201, Czech Republic

[6] Acad Sci Czech Republ, Inst Parasitol, Ctr Biol, Electron Microscopy Lab, CR-37005 Ceske Budejovice, Czech Republic

[7] Tech Univ Liberec, Dept Nonwovens, Liberec 46117, Czech Republic

来源：

BIOMACROMOLECULES | 2012年 / 13卷 / 04期

关键词：

CELL INFILTRATION; ELECTROSPUN NANOFIBERS; POLYMERIC NANOFIBERS; ENCAPSULATION; SCAFFOLDS; FIBER; RELEASE; MESHES; MICROFIBER; MATRIX;

D O I：

10.1021/bm2018118

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The broader application of liposomes in regenerative medicine is hampered by their short half-life and inefficient retention at the site of application. These disadvantages could be significantly reduced by their combination with nanofibers. We produced 2 different nanofiber-liposome systems in the present study, that is, liposomes blended within nanofibers and core/shell nanofibers with embedded liposomes. Herein, we demonstrate that blend electrospinning does not conserve intact liposomes. In contrast, coaxial electrospinning enables the incorporation of liposomes into nanofibers. We report polyvinyl alcohol-core/poly-epsilon-caprolactone-shell nanofibers with embedded liposomes and show that they preserve the enzymatic activity of encapsulated horseradish peroxidase. The potential of this system was also demonstrated by the enhancement of mesenchymal stem cell proliferation., In conclusion, intact liposomes incorporated into nanofibers by coaxial electrospinning are very promising as a drug delivery system.

引用

页码：952 / 962

页数：11

共 54 条

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