Batch crystallization of rhodopsin for structural dynamics using an X-ray free-electron laser

被引:12
作者
Wu, Wenting [1 ]
Nogly, Przemyslaw [1 ]
Rheinberger, Jan [1 ]
Kick, Leonhard M. [1 ]
Gati, Cornelius [1 ]
Nelson, Garrett [1 ]
Deupi, Xavier [1 ]
Standfuss, Joerg [1 ]
Schertler, Gebhard [1 ]
Panneels, Valerie [1 ]
机构
[1] Paul Scherrer Inst, Lab Biomol Res, CH-5232 Villigen, Switzerland
来源
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2015年 / 71卷
基金
瑞士国家科学基金会;
关键词
batch crystallization; GPCR; serial crystallography; FEL; dynamics; SERIAL FEMTOSECOND CRYSTALLOGRAPHY; PROTEIN-STRUCTURE DETERMINATION; CRYSTAL-STRUCTURE; COUPLED RECEPTOR; NANOCRYSTALLOGRAPHY; OPPORTUNITIES;
D O I
10.1107/S2053230X15009966
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Rhodopsin is a membrane protein from the G protein-coupled receptor family. Together with its ligand retinal, it forms the visual pigment responsible for night vision. In order to perform ultrafast dynamics studies, a time-resolved serial femtosecond crystallography method is required owing to the nonreversible activation of rhodopsin. In such an approach, microcrystals in suspension are delivered into the X-ray pulses of an X-ray free-electron laser (XFEL) after a precise photoactivation delay. Here, a millilitre batch production of high-density microcrystals was developed by four methodical conversion steps starting from known vapour-diffusion crystallization protocols: (i) screening the low-salt crystallization conditions preferred for serial crystallography by vapour diffusion, (ii) optimization of batch crystallization, (iii) testing the crystal size and quality using second-harmonic generation (SHG) imaging and X-ray powder diffraction and (iv) production of millilitres of rhodopsin crystal suspension in batches for serial crystallography tests; these crystals diffracted at an XFEL at the Linac Coherent Light Source using a liquid-jet setup.
引用
收藏
页码:856 / 860
页数:5
相关论文
共 50 条
  • [41] The collapse of a sonoluminescent cavitation bubble imaged with X-ray free-electron laser pulses
    Hoeppe, Hannes P.
    Osterhoff, Markus
    Maleki, Atiyeh Aghel
    Rossello, Juan M.
    Vassholz, Malte
    Hagemann, Johannes
    Engler, Thea
    Schwarz, Daniel
    Rodriguez-Fernandez, Angel
    Boesenberg, Ulrike
    Moeller, Johannes
    Shayduk, Roman
    Hallmann, Joerg
    Madsen, Anders
    Mettin, Robert
    Salditt, Tim
    NEW JOURNAL OF PHYSICS, 2024, 26 (03):
  • [42] Parallel Broadband Femtosecond Reflection Spectroscopy at a Soft X-Ray Free-Electron Laser
    Engel, Robin Y.
    Miedema, Piter S.
    Turenne, Diego
    Vaskivskyi, Igor
    Brenner, Gunter
    Dziarzhytski, Siarhei
    Kuhlmann, Marion
    Schunck, Jan O.
    Doring, Florian
    Styervoyedov, Andriy
    Parkin, Stuart S. P.
    David, Christian
    Schussler-Langeheine, Christian
    Durr, Hermann A.
    Beye, Martin
    APPLIED SCIENCES-BASEL, 2020, 10 (19): : 1 - 8
  • [43] Megahertz data collection from protein microcrystals at an X-ray free-electron laser
    Grunbein, Marie Luise
    Bielecki, Johan
    Gorel, Alexander
    Stricker, Miriam
    Bean, Richard
    Cammarata, Marco
    Doerner, Katerina
    Froehlich, Lars
    Hartmann, Elisabeth
    Hauf, Steffen
    Hilpert, Mario
    Kim, Yoonhee
    Kloos, Marco
    Letrun, Romain
    Messerschmidt, Marc
    Mills, Grant
    Kovacs, Gabriela Nass
    Ramilli, Marco
    Roome, Christopher M.
    Sato, Tokushi
    Scholz, Matthias
    Sliwa, Michel
    Sztuk-Dambietz, Jolanta
    Weik, Martin
    Weinhausen, Britta
    Al-Qudami, Nasser
    Boukhelef, Djelloul
    Brockhauser, Sandor
    Ehsan, Wajid
    Emons, Moritz
    Esenov, Sergey
    Fangohr, Hans
    Kaukher, Alexander
    Kluyver, Thomas
    Lederer, Max
    Maia, Luis
    Manetti, Maurizio
    Michelat, Thomas
    Muennich, Astrid
    Pallas, Florent
    Palmer, Guido
    Previtali, Gianpietro
    Raab, Natascha
    Silenzi, Alessandro
    Szuba, Janusz
    Venkatesan, Sandhya
    Wrona, Krzysztof
    Zhu, Jun
    Doak, R. Bruce
    Shoeman, Robert L.
    NATURE COMMUNICATIONS, 2018, 9
  • [44] Attosecond time-energy structure of X-ray free-electron laser pulses
    Hartmann, N.
    Hartmann, G.
    Heider, R.
    Wagner, M. S.
    Ilchen, M.
    BucK, J.
    Lindahl, A. O.
    Benko, C.
    Gruenert, J.
    Krzywinski, J.
    Liu, J.
    Lutman, A. A.
    Marinelli, A.
    Maxwell, T.
    Miahnahri, A. A.
    Moeller, S. P.
    Planas, M.
    Robinson, J.
    Kazansky, A. K.
    Kabachnik, N. M.
    Viefhaus, J.
    Feurer, T.
    Kienberger, R.
    Coffee, R. N.
    Helml, W.
    NATURE PHOTONICS, 2018, 12 (04) : 215 - +
  • [45] Reverse taper enhanced harmonic lasing for seeding an X-ray free-electron laser
    Zhang, Kaiqing
    Liu, Tao
    Feng, Chao
    NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 2021, 988
  • [46] Biomolecular imaging and electronic damage using X-ray free-electron lasers
    Quiney, Harry M.
    Nugent, Keith A.
    NATURE PHYSICS, 2011, 7 (02) : 142 - 146
  • [47] Membrane protein structural biology using X-ray free electron lasers
    Neutze, Richard
    Braenden, Gisela
    Schertler, Gebhard F. X.
    CURRENT OPINION IN STRUCTURAL BIOLOGY, 2015, 33 : 115 - 125
  • [48] Pink-beam serial femtosecond crystallography for accurate structure-factor determination at an X-ray free-electron laser
    Nass, Karol
    Bacellar, Camila
    Cirelli, Claudio
    Dworkowski, Florian
    Gevorkov, Yaroslav
    James, Daniel
    Johnson, Philip J. M.
    Kekilli, Demet
    Knopp, Gregor
    Martiel, Isabelle
    Ozerov, Dmitry
    Tolstikova, Alexandra
    Vera, Laura
    Weinert, Tobias
    Yefanov, Oleksandr
    Standfuss, Joerg
    Reiche, Sven
    Milne, Christopher J.
    IUCRJ, 2021, 8 : 905 - 920
  • [49] Multi-wavelength anomalous diffraction de novo phasing using a two-colour X-ray free-electron laser with wide tunability
    Gorel, Alexander
    Motomura, Koji
    Fukuzawa, Hironobu
    Doak, R. Bruce
    Gruenbein, Marie Luise
    Hilpert, Mario
    Inoue, Ichiro
    Kloos, Marco
    Kovacsova, Gabriela
    Nango, Eriko
    Nass, Karol
    Roome, Christopher M.
    Shoeman, Robert L.
    Tanaka, Rie
    Tono, Kensuke
    Joti, Yasumasa
    Yabashi, Makina
    Iwata, So
    Foucar, Lutz
    Ueda, Kiyoshi
    Barends, Thomas R. M.
    Schlichting, Ilme
    NATURE COMMUNICATIONS, 2017, 8
  • [50] X-ray free-electron laser based dark-field X-ray microscopy: a simulation-based study
    Holstad, Theodor Secanell
    Raeder, Trygve Magnus
    Carlsen, Mads
    Knudsen, Erik Bergback
    Dresselhaus-Marais, Leora
    Haldrup, Kristoffer
    Simons, Hugh
    Nielsen, Martin Meedom
    Poulsen, Henning Friis
    JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2022, 55 : 112 - 121