Membrane labeling and immobilization via copper-free click chemistry

被引：19

作者：

Bostic, Heidi E. ^{[1
]}

Smith, Matthew D. ^{[1
]}

Poloukhtine, Andrei A. ^{[2
]}

Popik, Vladimir V. ^{[2
]}

Best, Michael D. ^{[1
]}

机构：

[1] Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA

[2] Univ Georgia, Dept Chem, Athens, GA 30602 USA

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 10期

基金：

美国国家科学基金会;

关键词：

AZIDE-ALKYNE CYCLOADDITION; BIOORTHOGONAL CHEMISTRY; BINDING INTERACTIONS; TERMINAL ALKYNES; DRUG-DELIVERY; SELECTIVITY; LIGANDS; MOLECULES; LIPOSOMES; CATALYSTS;

D O I：

10.1039/c1cc14415d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Copper-free click chemistry was employed to derivatize membrane bilayers. This approach uses an azido-lipid conjugate presented on liposomes, which can be labeled in bioorthogonal fashion via cyclooctyne-tagged reagents. An immobilization-based approach using streptavidin-coated microplates was exploited to evaluate membrane derivatization.

引用

页码：1431 / 1433

页数：3

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