Allosteric Molecular Beacons for Sensitive Detection of Nucleic Acids, Proteins, and Small Molecules in Complex Biological Samples

被引：28

作者：

Song, Yanling ^{[1
,2
]}

Cui, Liang ^{[1
,2
]}

Wu, Jie ^{[1
,2
]}

Zhang, Weiting ^{[1
,2
]}

Zhang, Wei Yun ^{[1
,2
]}

Kang, Huaizhi ^{[1
,2
]}

Yang, Chaoyong James ^{[1
,2
]}

机构：

[1] Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Key Lab Chem Biol Fujian Prov, Key Lab Analyt Sci, Xiamen 361005, Peoples R China

[2] Xiamen Univ, Dept Biol Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2011年 / 17卷 / 33期

基金：

美国国家科学基金会;

关键词：

allosteric probes; aptamers; DNA; fluorescent probes; proteins; molecular beacons; QUENCHED FLUORESCENT OLIGONUCLEOTIDES; EXCIMER PROBES; DNA; HYBRIDIZATION; FLUIDS; RNA;

D O I：

10.1002/chem.201101353

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Beacon of light! A new type of probe called an allosteric molecular beacon (aMB) has been designed for highly sensitive detection of nucleic acids, proteins, and small molecules. The aMB is designed in such way that target binding will activate its binding affinity to streptavidin microbeads for probe signal enrichment, noise reduction, and signal readout, which enables ultrasensitive target detection in complex biological samples (see scheme). © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9042 / 9046

页数：5

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