Isolation and characterisation of a new antimicrobial peptide from the skin of Xenopus laevis

被引：43

作者：

Hou, Feng ^{[1
,2
]}

Li, Jiping ^{[1
,2
]}

Pan, Pengpeng ^{[1
,2
]}

Xu, Jing ^{[1
,2
]}

Liu, Linna ^{[1
,2
]}

Liu, Wensen ^{[1
,2
]}

Song, Bocui ^{[1
,2
]}

Li, Nan ^{[1
,2
]}

Wan, Jiayu ^{[1
,2
]}

Gao, Hongwei ^{[1
,2
]}

机构：

[1] Acad Mil Med Sci, Inst Mil Vet Sci, 666 Liuying W Rd, Changchun 130062, Jilin, Peoples R China

[2] Key Lab Jilin Prov Zoonoses Prevent & Control, Changchun 130062, Jilin, Peoples R China

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2011年 / 38卷 / 06期

关键词：

Antimicrobial peptides; Xenopus laevis; Skin; PGLa-H; SECRETIONS; FROGS; TOAD;

D O I：

10.1016/j.ijantimicag.2011.07.012

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A new antimicrobial peptide (AMP) named PGLa-H has been isolated from the skin of the African clawed frog (Xenopus laevis) using gel filtration and reverse-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as KIAKVALKAL by Edman degradation, with a molecular weight of 1053.727 Da as analysed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). No similar AMP was found by BLAST search. Purified PGLa-H demonstrated antimicrobial ability against the reference bacteria Escherichia coli ATCC 25922 [minimum inhibitory concentration (MIC) = 23.6 mu g/mL], Staphylococcus aureus ATCC 25923 (MIC = 8.7 mu g/mL) and Bacillus subtilis (MIC = 14.4 mu g/mL) and was active against multidrug-resistant meticillin-resistant S. aureus (MRSA) (MIC = 67.8 mu g/mL). The antimicrobial mechanism for this new peptide was further investigated by transmission electron microscopy. PGLa-H killed cells by destroying the cell membrane. (C) 2011 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.

引用

页码：510 / 515

页数：6

共 17 条

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