Marinobacter arenosus sp. nov., a halotolerant bacterium isolated from a tidal flat

A Gram-negative, rod-shaped, motile bacterium, designated strain CAU 1620(T), was isolated from a tidal flat sediment in Incheon, Republic of Korea. Strain CAU 1620(T) grew optimally at 30 degrees C and pH 8.0 in the presence of 6.0% (w/v) NaCl. The results of 16S rRNA gene sequence analysis revealed that strain CAU 1620(T) showed the highest similarity to Marinobacter adhaerens DSM 23420(T) (98.5%), followed by Marinobacter algicola DSM 16394(T) (98.3%) and Marinobacter maroccanus LMG 30465(T) (98.2%). The average nucleotide identity and digital DNA-DNA hybridisation values between strain CAU 1620(T) and related strains were estimated as 75.6-78.1% and 19.5-20.9%, respectively. The DNA G + C content based on the draft genome sequence was 59.2%, and the major respiratory quinone was ubiquinone-9. The predominant cellular fatty acids were C-12:0,C- C-16:0,C- C-18:1 omega 9c, and C-12:0 3OH. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. According to phenotypic, genotypic, and chemotaxonomic analyses, strain CAU 1620(T) represents a novel species of the genus Marinobacter, for which the name Marinobacter arenosus sp. nov. is proposed. The type strain is CAU 1620(T) (= KCTC 82431(T) = MCCC 1K06079(T)).