Quantification of lupus anticoagulants in clinical samples using anti-β2GP1 and anti-prothrombin monoclonal antibodies

被引:0
|
作者
Le Querrec, A
Arnout, J
Arnoux, D
Borg, JY
Caron, C
Darnige, L
Delahousse, B
Reber, G
Sié, P
机构
[1] Hop Cote de Nacre, Caen, France
[2] Ctr Mol & Vasc Biol, Louvain, Belgium
[3] Hop La Concept, Marseille, France
[4] Hop Charles Nicolle, Rouen, France
[5] Hop Cardiol, Lille, France
[6] Hop Compiegne, Compiegne, France
[7] Hop Trousseau, Tours, France
[8] Univ Geneva, Hop Cantonal, CH-1211 Geneva, Switzerland
[9] Hop Purpan, Toulouse, France
关键词
lupus anticoagulant; anti-beta(2)glycoprotein 1; anti-prothrombin; monoclonal antibodies; antiphospholipid syndrome;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Quantification of lupus anticoagulant (LA) in clinical samples is hampered by the lack of a suitable standard of activity. We evaluated the use of mAbs displaying LA activity for this purpose. As most patient samples contain both beta (2)Glycoprotein I (beta (2)Gpl) and prothrombin dependent LA, a combination of two mAbs, one of each specificity, was added to normal plasma in a concentration from 0 to 60 mug/ml. Eight assay systems using different reagents and instruments were used. The calibration curves were linear for all but one, with marked differences between the responsiveness to each mAb. A panel of plasmas from 69 patients with persistent LA diagnosed using the SSC-ISTH criteria was tested. An antiphospholipid syndrome (APS) was present in 40, whereas 29 were asymptomatic. LA activities of individual plasmas varied between assays (p < 10(-4)), but homogeneous suba groups were identified. In a majority of samples, LA activity displayed a prothrombin-dependent profile, with a variable contribution of beta (2)GPl-dependent activity. The latter was associated to P(2)GPl antibodies detected by solid-phase immunoassay. By using 3 dilute Russell viper venom time assays, higher LA titers were found in APS, compared to asymptomatic patients (p <0.05).
引用
收藏
页码:584 / 589
页数:6
相关论文
共 50 条
  • [31] Anti-β2-glycoprotein I and anti-phosphatidylserine/prothrombin antibodies interfere with cleavage of factor V(a) by activated protein C
    Noordermeer, Tessa
    Chemlal, Soumaya
    Jansma, Janna J.
    van der Vegte, Vossa
    Schutgens, Roger E. G.
    Limper, Maarten
    de Groot, Philip G.
    Meijers, Joost C. M.
    Urbanus, Rolf T.
    JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2023, 21 (09) : 2509 - 2518
  • [32] Clinical Significance of IgA Anti-Cardiolipin and IgA Anti-β2Glycoprotein I Antibodies
    Laura Andreoli
    Micaela Fredi
    Cecilia Nalli
    Silvia Piantoni
    Rossella Reggia
    Francesca Dall’Ara
    Franco Franceschini
    Angela Tincani
    Current Rheumatology Reports, 2013, 15
  • [33] Anti-β2-glycoprotein I antibodies in pediatric systemic lupus erythematosus and antiphospholipid syndrome
    von Scheven, E
    Glidden, DV
    Elder, ME
    ARTHRITIS & RHEUMATISM-ARTHRITIS CARE & RESEARCH, 2002, 47 (04): : 414 - 420
  • [34] Anticardiolipin and anti-β2GPI antibodies in a large series of European patients with systemic lupus erythematosus -: Prevalence and clinical associations
    Sebastiani, GD
    Galeazzi, M
    Tincani, A
    Piette, JC
    Font, J
    Allegri, F
    Mathieu, A
    Smolen, J
    Garrido, ED
    Fernandez-Nebro, A
    Jedryka-Goral, A
    Papasteriades, C
    Morozzi, G
    Bellisai, F
    De Pita, O
    Marcolongo, R
    SCANDINAVIAN JOURNAL OF RHEUMATOLOGY, 1999, 28 (06) : 344 - 351
  • [35] IgG anti-β2 glycoprotein I antibodies in Malaysian patients with antiphospholipid syndrome and systemic lupus erythematosus:: Prevalence and clinical correlations
    Ong, SG
    Cheng, HM
    Soon, SC
    Goh, E
    Chow, SK
    Yeap, SS
    CLINICAL RHEUMATOLOGY, 2002, 21 (05) : 382 - 385
  • [36] The persistence of anticardiolipin antibodies is associated with an increased risk of the presence of lupus anticoagulant and anti-β2-glycoprotein I antibodies
    Neville, C.
    Rauch, J.
    Kassis, J.
    Solymoss, S.
    Joseph, L.
    Belisle, P.
    Subang, R.
    Chang, E. R.
    Fortin, P. R.
    RHEUMATOLOGY, 2006, 45 (09) : 1116 - 1120
  • [37] IgG antibodies to plasminogen and their relationship to IgG anti-β2-glycoprotein 1 antibodies and thrombosis
    Bu, Chunya
    Li, Zhihuan
    Zhang, Chune
    Gao, Lei
    Cai, Guoping
    CLINICAL RHEUMATOLOGY, 2008, 27 (02) : 171 - 178
  • [38] IgG antibodies to plasminogen and their relationship to IgG anti-β2-glycoprotein 1 antibodies and thrombosis
    Chunya Bu
    Zhihuan Li
    Chune Zhang
    Lei Gao
    Guoping Cai
    Clinical Rheumatology, 2008, 27 : 171 - 178
  • [39] Isotypes of anti-β2-glycoprotein I antibodies:: Association with thrombosis in patients with systemic lupus erythematosus
    Lee, SS
    Cho, ML
    Joo, YS
    Kim, WU
    Hong, YS
    Min, JK
    Lee, SH
    Park, SH
    Cho, CS
    Kim, HY
    JOURNAL OF RHEUMATOLOGY, 2001, 28 (03) : 520 - 524
  • [40] IgG anti-β2-glycoprotein I antibodies in adult patients with systemic lupus erythematosus:: Prevalence and diagnostic value for the antiphospholipid syndrome
    Tubach, F
    Hayem, G
    Marchand, JL
    Weber, M
    Palazzo, E
    de Bandt, M
    Roux, S
    Kahn, MF
    Meyer, O
    JOURNAL OF RHEUMATOLOGY, 2000, 27 (06) : 1437 - 1443