Quantification of lupus anticoagulants in clinical samples using anti-β2GP1 and anti-prothrombin monoclonal antibodies

被引:0
|
作者
Le Querrec, A
Arnout, J
Arnoux, D
Borg, JY
Caron, C
Darnige, L
Delahousse, B
Reber, G
Sié, P
机构
[1] Hop Cote de Nacre, Caen, France
[2] Ctr Mol & Vasc Biol, Louvain, Belgium
[3] Hop La Concept, Marseille, France
[4] Hop Charles Nicolle, Rouen, France
[5] Hop Cardiol, Lille, France
[6] Hop Compiegne, Compiegne, France
[7] Hop Trousseau, Tours, France
[8] Univ Geneva, Hop Cantonal, CH-1211 Geneva, Switzerland
[9] Hop Purpan, Toulouse, France
关键词
lupus anticoagulant; anti-beta(2)glycoprotein 1; anti-prothrombin; monoclonal antibodies; antiphospholipid syndrome;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Quantification of lupus anticoagulant (LA) in clinical samples is hampered by the lack of a suitable standard of activity. We evaluated the use of mAbs displaying LA activity for this purpose. As most patient samples contain both beta (2)Glycoprotein I (beta (2)Gpl) and prothrombin dependent LA, a combination of two mAbs, one of each specificity, was added to normal plasma in a concentration from 0 to 60 mug/ml. Eight assay systems using different reagents and instruments were used. The calibration curves were linear for all but one, with marked differences between the responsiveness to each mAb. A panel of plasmas from 69 patients with persistent LA diagnosed using the SSC-ISTH criteria was tested. An antiphospholipid syndrome (APS) was present in 40, whereas 29 were asymptomatic. LA activities of individual plasmas varied between assays (p < 10(-4)), but homogeneous suba groups were identified. In a majority of samples, LA activity displayed a prothrombin-dependent profile, with a variable contribution of beta (2)GPl-dependent activity. The latter was associated to P(2)GPl antibodies detected by solid-phase immunoassay. By using 3 dilute Russell viper venom time assays, higher LA titers were found in APS, compared to asymptomatic patients (p <0.05).
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收藏
页码:584 / 589
页数:6
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