Establishment of rat anti-canine DEP domain containing 1B (DEPDC1B) monoclonal antibodies

被引：2
作者：
Igase, Masaya [1 ]
Morinaga, Yuki [1 ]
Kato, Masahiro [2 ]
Tsukui, Toshihiro [2 ]
Sakai, Yusuke [3 ]
Okuda, Masaru [4 ]
Mizuno, Takuya [1 ]
机构：
[1] Yamaguchi Univ, Joint Fac Vet Med, Lab Mol Diagnost & Therapeut, 1677-1 Yoshida, Yamaguchi, Yamaguchi 7538511, Japan
[2] Nippon Zenyaku Kogyo Co Ltd, 1-1 Tairanoue, Koriyama, Fukushima 9630196, Japan
[3] Yamaguchi Univ, Joint Fac Vet Med, Lab Vet Pathol, 1677-1 Yoshida, Yamaguchi, Yamaguchi 7538511, Japan
[4] Yamaguchi Univ, Joint Fac Vet Med, Lab Vet Internal Med, 1677-1 Yoshida, Yamaguchi, Yamaguchi 7538511, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2020年 / 82卷 / 04期
关键词：
antibody; cancer; DEP domain-containing 1B; dog; CELL-DEATH; ORGANIZATION; EXPRESSION; RHO;
D O I：
10.1292/jvms.19-0667
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
DEP domain-containing 1B (DEPDC1B) is involved in the regulation of cell deadhesion and actin cytoskeleton activity during the G2/M transition of the cell cycle, and its overexpression has been proven to be associated with cancer progression in several human cancers. Canine DEPDC1B was identified as a gene that was overexpressed in canine lymphoma tissues in our previous study. However, in dogs, the protein expression of DEPDC1B remains to be determined due to the lack of a specific monoclonal antibody. Here, we developed rat monoclonal antibodies against canine DEPDC1B and characterized their applicability for immunodetection assays. Our findings demonstrated that these antibodies are functional and can be important tools to investigate the precise role of DEPDC1B in canine tumors.
引用
收藏
页码：483 / 487
页数：5
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