Determinations of morin, quercetin and their conjugate metabolites in serum

被引：30
作者：
Hsiu, SL
Tsao, CW
Tsai, YC
Ho, HJ
Chao, PDL [1 ]
机构：
[1] China Med Coll, Sch Pharm, Taichung 404, Taiwan
[2] China Med Coll, Grad Inst Pharmaceut Chem, Taichung 404, Taiwan
[3] China Med Coll, Grad Inst Chinese Pharmaceut Sci, Taichung 404, Taiwan
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2001年 / 24卷 / 08期
关键词：
morin; quercetin; serum; HPLC;
D O I：
10.1248/bpb.24.967
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Morin and quercetin are isomeric antioxidant flavonols. High-performance liquid chromatographic methods were developed for the quantitation of morin and quercetin in serum. The method employed a Cosmosil RP-18 column, using acetonitrile/0.2% o-phosphoric acid: 28/72 and 27/73 (v/v) as mobile phases, with ethyl paraben and 6,7-dimethoxycoumarin used as internal standards for morin and quercetin, respectively. Moreover, a strategy to stabilize morin/quercetin released from their glucuronides/sulfates in serum during hydrolysis was established. The present methods are applicable for determining morin, quercetin, and their glucuronides/sulfates in serum.
引用
收藏
页码：967 / 969
页数：3
相关论文
共 15 条
[1]   CHEMOPREVENTIVE POTENTIAL OF DIETARY BIOFLAVONOIDS AGAINST 20-METHYLCHOLANTHRENE-INDUCED TUMORIGENESIS [J].
ELANGOVAN, V ;
SEKAR, N ;
GOVINDASAMY, S .
CANCER LETTERS, 1994, 87 (01) :107-113
[2]  
Ferry DR, 1996, CLIN CANCER RES, V2, P659
[3]   FREE-RADICALS, ANTIOXIDANTS, AND HUMAN-DISEASE - CURIOSITY, CAUSE, OR CONSEQUENCE [J].
HALLIWELL, B .
LANCET, 1994, 344 (8924) :721-724
[4]   Absorption and disposition kinetics of the dietary antioxidant quercetin in man [J].
Hollman, PCH ;
vanderGaag, M ;
Mengelers, MJB ;
vanTrijp, JMP ;
deVries, JH ;
Katan, MB .
FREE RADICAL BIOLOGY AND MEDICINE, 1996, 21 (05) :703-707
[5]  
LEIGHTON T, 1992, PHENOLIC COMPOUNDS F, V1, P220
[6]   Bioavailability of rutin and quercetin in rats [J].
Manach, C ;
Morand, C ;
Demigne, C ;
Texier, O ;
Regerat, F ;
Remesy, C .
FEBS LETTERS, 1997, 409 (01) :12-16
[7]   Bioavailability, metabolism and physiological impact of 4-oxo-flavonoids [J].
Manach, C ;
Regerat, F ;
Texier, O ;
Agullo, G ;
Demigne, C ;
Remesy, C .
NUTRITION RESEARCH, 1996, 16 (03) :517-544
[8]   Antioxidant properties of flavonol glycosides from green beans [J].
Plumb, GW ;
Price, KR ;
Williamson, G .
REDOX REPORT, 1999, 4 (03) :123-127
[9]   STUDIES ON LIPID OXIDATION IN FISH PHOSPHOLIPID LIPOSOMES [J].
RAMANATHAN, L ;
DAS, NP ;
LI, QT .
BIOLOGICAL TRACE ELEMENT RESEARCH, 1994, 40 (01) :59-70
[10]   QUERCETIN INHIBITS THE GROWTH OF A MULTIDRUG-RESISTANT ESTROGEN-RECEPTOR-NEGATIVE MCF-7 HUMAN BREAST-CANCER CELL-LINE EXPRESSING TYPE-II ESTROGEN-BINDING SITES [J].
SCAMBIA, G ;
RANELLETTI, FO ;
PANICI, PB ;
PIANTELLI, M ;
BONANNO, G ;
DEVINCENZO, R ;
FERRANDINA, G ;
PIERELLI, L ;
CAPELLI, A ;
MANCUSO, S .
CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1991, 28 (04) :255-258
12