Determinations of morin, quercetin and their conjugate metabolites in serum

被引：30
|
作者：
Hsiu, SL
Tsao, CW
Tsai, YC
Ho, HJ
Chao, PDL [1 ]
机构：
[1] China Med Coll, Sch Pharm, Taichung 404, Taiwan
[2] China Med Coll, Grad Inst Pharmaceut Chem, Taichung 404, Taiwan
[3] China Med Coll, Grad Inst Chinese Pharmaceut Sci, Taichung 404, Taiwan
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2001年 / 24卷 / 08期
关键词：
morin; quercetin; serum; HPLC;
D O I：
10.1248/bpb.24.967
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Morin and quercetin are isomeric antioxidant flavonols. High-performance liquid chromatographic methods were developed for the quantitation of morin and quercetin in serum. The method employed a Cosmosil RP-18 column, using acetonitrile/0.2% o-phosphoric acid: 28/72 and 27/73 (v/v) as mobile phases, with ethyl paraben and 6,7-dimethoxycoumarin used as internal standards for morin and quercetin, respectively. Moreover, a strategy to stabilize morin/quercetin released from their glucuronides/sulfates in serum during hydrolysis was established. The present methods are applicable for determining morin, quercetin, and their glucuronides/sulfates in serum.
引用
收藏
页码：967 / 969
页数：3
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