Whole genome sequencing of carbapenem-resistant Klebsiella pneumoniae: evolutionary analysis for outbreak investigation

被引：6
|
作者：
Fontana, Carla [1 ,2 ]
Angeletti, Silvia [3 ]
Mirandola, Walter [1 ]
Cella, Eleonora [4 ]
Alessias, Lai [5 ]
Zehender, Gianguglielmo [5 ]
Favaro, Marco [2 ]
Leoni, Davide [6 ]
Delle Rose, Diego [6 ]
Gherardi, Giovanni [3 ]
De Florio, Lucia [3 ]
Salemi, Marco [7 ,8 ]
Andreoni, Massimo [6 ]
Sarmati, Loredana [6 ]
Ciccozzi, Massimo [4 ]
机构：
[1] Polyclin Tor Vergata, Clin Microbiol Labs, Rome, Italy
[2] Univ Roma Tor Vergata, Dept Expt Med & Biochem Sci, Rome, Italy
[3] Univ Campus Biomed Rome, Unit Clin Lab Sci, Rome, Italy
[4] Univ Campus Biomed Rome, Unit Med Stat & Mol Epidemiol, Rome, Italy
[5] Univ Milan, Dept Biomed & Clin Sci L Sacco, Milan, Italy
[6] Tor Vergata Univ, Dept Syst Med, Clin Infect Dis, Rome, Italy
[7] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32610 USA
[8] Univ Florida, Dept Pathol Immunol & Lab Med, Gainesville, FL 32610 USA
来源：
FUTURE MICROBIOLOGY | 2020年 / 15卷 / 03期
关键词：
Klebsiella pneumoniae; carabapenemase resistance; nosocomial infection; phylogenetic analysis; whole genome sequencing; HYDROLYZING BETA-LACTAMASE; KPC; ENTEROBACTERIACEAE; EPIDEMIOLOGY; SPREAD; IDENTIFICATION; DISSEMINATION; PLASMIDS;
D O I：
10.2217/fmb-2019-0074
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: Carbapenemase-resistant Enterobacteriaceae represents a major concern in hospital setting. Materials & methods: The evolutionary history of carbapenem-resistant Klebsiella pneumonia strains was analyzed by core genome multilocus sequence typing and Bayesian phylogenesis by whole genomes sequencing. Results: A great increase carbapenem-resistant K.pneumoniae causing blood stream infection was observed in the years 2015-2016. At multilocus sequence typing (MLST), they were prevalently ST512 and ST101. ST512 were core genome (cg)MLST 53, while ST101 mainly cgMLST453. The minimum-spanning tree, based on cgMLST, showed strains clustering based on the different STs. By Bayesian phylogenetic analysis, maximum clade credibility tree showed that strains were introduced in the year 2005 with the most probable location in the ICU ward. Two outbreaks by ST101 and ST512 strains with Tower T8 as the probable location were evidenced. Conclusion: Molecular epidemiology is a powerful tool to track the way of transmission of resistant bacteria within the hospital setting.
引用
收藏
页码：203 / 212
页数：10
相关论文
共 50 条
  • [21] Genomic analysis of the initial dissemination of carbapenem-resistant Klebsiella pneumoniae clones in a tertiary hospital
    Garcia-Gonzalez, Neris
    Fuster, Begona
    Tormo, Nuria
    Salvador, Carme
    Gimeno, Concepcion
    Gonzalez-Candelas, Fernando
    MICROBIAL GENOMICS, 2023, 9 (06):
  • [22] Epigenomics, genomics, resistome, mobilome, virulome and evolutionary phylogenomics of carbapenem-resistant Klebsiella pneumoniae clinical strains
    Kopotsa, Katlego
    Mbelle, Nontombi M.
    Sekyere, John Osei
    MICROBIAL GENOMICS, 2020, 6 (12): : 1 - 19
  • [23] Epidemiological study of carbapenem-resistant Klebsiella pneumoniae
    Lin, Di
    Chen, Jian
    Yang, Yan
    Cheng, Jun
    Sun, Changgui
    OPEN MEDICINE, 2018, 13 (01): : 460 - 466
  • [24] Reducing dissemination of carbapenem-resistant Klebsiella pneumoniae
    Bassetti, Matteo
    Giacobbe, Daniele Roberto
    ANNALS OF TRANSLATIONAL MEDICINE, 2019, 7
  • [25] Risk Factors for the Acquisition of Nosocomial Infection with Carbapenem-Resistant Klebsiella pneumoniae
    Wu, Dandan
    Cai, Jiachang
    Liu, Jin
    SOUTHERN MEDICAL JOURNAL, 2011, 104 (02) : 106 - 110
  • [26] Risk Factors for Subsequential Carbapenem-Resistant Klebsiella pneumoniae Clinical Infection Among Rectal Carriers with Carbapenem-Resistant Klebsiella pneumoniae
    Chen, Xia
    Liu, Qingnuan
    Liu, Wen-en
    Yan, Qun
    INFECTION AND DRUG RESISTANCE, 2020, 13 : 1299 - 1305
  • [27] Epidemiology of the first outbreak of carbapenem-resistant Klebsiella pneumoniae in Saudi Arabia
    HH Balkhy
    A El-Saed
    S Al Johani
    HT Tayeb
    A Al-Qahtani
    M Alahdal
    M Sallah
    A Alothman
    Y Alarabi
    BMC Proceedings, 5 (Suppl 6)
  • [28] Genomic Analysis of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae in a Chinese Tertiary Hospital
    Chen, Lan
    Zhou, Ying
    Wang, Shanshan
    Wu, Chunyang
    Zhou, Peiyao
    Wang, Bingjie
    Chen, Zhu
    Yu, Fangyou
    INFECTION AND DRUG RESISTANCE, 2023, 16 : 6385 - 6394
  • [29] Outbreak of Carbapenem-Resistant Klebsiella pneumoniae in Puerto Rico Associated with a Novel Carbapenemase Variant
    Gregory, Christopher J.
    Llata, Eloisa
    Stine, Nicholas
    Gould, Carolyn
    Santiago, Luis Manuel
    Vazquez, Guillermo J.
    Robledo, Iraida E.
    Srinivasan, Arjun
    Goering, Richard V.
    Tomashek, Kay M.
    INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 2010, 31 (05) : 476 - 484
  • [30] Managing a nosocomial outbreak of carbapenem-resistant Klebsiella pneumoniae: an early Australian hospital experience
    Chang, L. W. K.
    Buising, K. L.
    Jeremiah, C. J.
    Cronin, K.
    Lorenzo, Y. S. Poy
    Howden, B. P.
    Kwong, J.
    Cocks, J.
    Blood, A.
    Greenough, J.
    Waters, M. J.
    INTERNAL MEDICINE JOURNAL, 2015, 45 (10) : 1037 - 1043
12345