Whole genome sequencing of carbapenem-resistant Klebsiella pneumoniae: evolutionary analysis for outbreak investigation

被引：6
|
作者：
Fontana, Carla [1 ,2 ]
Angeletti, Silvia [3 ]
Mirandola, Walter [1 ]
Cella, Eleonora [4 ]
Alessias, Lai [5 ]
Zehender, Gianguglielmo [5 ]
Favaro, Marco [2 ]
Leoni, Davide [6 ]
Delle Rose, Diego [6 ]
Gherardi, Giovanni [3 ]
De Florio, Lucia [3 ]
Salemi, Marco [7 ,8 ]
Andreoni, Massimo [6 ]
Sarmati, Loredana [6 ]
Ciccozzi, Massimo [4 ]
机构：
[1] Polyclin Tor Vergata, Clin Microbiol Labs, Rome, Italy
[2] Univ Roma Tor Vergata, Dept Expt Med & Biochem Sci, Rome, Italy
[3] Univ Campus Biomed Rome, Unit Clin Lab Sci, Rome, Italy
[4] Univ Campus Biomed Rome, Unit Med Stat & Mol Epidemiol, Rome, Italy
[5] Univ Milan, Dept Biomed & Clin Sci L Sacco, Milan, Italy
[6] Tor Vergata Univ, Dept Syst Med, Clin Infect Dis, Rome, Italy
[7] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32610 USA
[8] Univ Florida, Dept Pathol Immunol & Lab Med, Gainesville, FL 32610 USA
来源：
FUTURE MICROBIOLOGY | 2020年 / 15卷 / 03期
关键词：
Klebsiella pneumoniae; carabapenemase resistance; nosocomial infection; phylogenetic analysis; whole genome sequencing; HYDROLYZING BETA-LACTAMASE; KPC; ENTEROBACTERIACEAE; EPIDEMIOLOGY; SPREAD; IDENTIFICATION; DISSEMINATION; PLASMIDS;
D O I：
10.2217/fmb-2019-0074
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: Carbapenemase-resistant Enterobacteriaceae represents a major concern in hospital setting. Materials & methods: The evolutionary history of carbapenem-resistant Klebsiella pneumonia strains was analyzed by core genome multilocus sequence typing and Bayesian phylogenesis by whole genomes sequencing. Results: A great increase carbapenem-resistant K.pneumoniae causing blood stream infection was observed in the years 2015-2016. At multilocus sequence typing (MLST), they were prevalently ST512 and ST101. ST512 were core genome (cg)MLST 53, while ST101 mainly cgMLST453. The minimum-spanning tree, based on cgMLST, showed strains clustering based on the different STs. By Bayesian phylogenetic analysis, maximum clade credibility tree showed that strains were introduced in the year 2005 with the most probable location in the ICU ward. Two outbreaks by ST101 and ST512 strains with Tower T8 as the probable location were evidenced. Conclusion: Molecular epidemiology is a powerful tool to track the way of transmission of resistant bacteria within the hospital setting.
引用
收藏
页码：203 / 212
页数：10
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