Voltametric measurement of blood nitric oxide in irradiated rats

被引:13
作者
Clarençon, D
Lestaevel, P
Laval, JD
Multon, E
Gourmelon, P
Buguet, A
Cespuglio, R
机构
[1] Ctr Rech Serv Sante Armees Emile Parde, Dept Radiobiol, Unite Radioprotect, F-38702 La Tronche, France
[2] Univ Lyon 1, INSERM U480, Dept Expt Med, F-69373 Lyon 08, France
[3] IPSN, Inst Protect & Surete Nucl, Dept Protect Sante Homme & Dosimetrie, Sect Autonome Radiobiol Appliquee Med, F-92265 Fontenay Aux Roses, France
关键词
D O I
10.1080/095530099140654
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Purpose: To investigate the effect of blood nitric oxide (NO) as a mediator of the neurovascular syndrome in rats following gamma-irradiation. Material and methods: Using a voltametric method together. with a carbon fibre based sensor, NO measurements were carried out in sham-irradiated and irradiated animals either in blood from the: abdominal aorta or in blood samples from the heart. Results: In in vitro conditions, properties of the probe were not altered by the ionizing radiation. Significant increases of +17% and + 25.6% were observed in the voltametric signal height at 90 min and 24 h respectively after a 15 Gy gamma-ray exposure. These effects were followed on days 3 and 4 by a progressive decrease in the signal height of 7% and 18% respectively. Dose-effect relationships were observed at 90 min and 24-h after exposure to gamma-rays in the. range of 3-15 Gy. Finally, the NO dependence on the measured voltametric signal was controlled by using inhibitors of the NO synthase (NOS) and by performing nitrate assays. Conclusions: Specific blood NO voltametric measurements are possible. Functional changes associated with NO after gamma-ray exposure are discussed.
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页码:201 / 208
页数:8
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