The p53/miR-34a/SIRT1 Positive Feedback Loop in Quercetin-Induced Apoptosis

被引：92

作者：

Lou, Guohua ^{[1
]}

Liu, Yanning ^{[1
]}

Wu, Shanshan ^{[1
]}

Xue, Jihua ^{[1
]}

Yang, Fan ^{[1
]}

Fu, Haijing ^{[1
]}

Zheng, Min ^{[1
]}

Chen, Zhi ^{[1
]}

机构：

[1] Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Affiliated Hosp 1, State Key Lab Diag & Treatment Infect Dis, Hangzhou 310003, Zhejiang, Peoples R China

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2015年 / 35卷 / 06期

关键词：

Quercetin; P53; MiR-34a; HCC; SIRT1; CANCER; MICRORNAS; MIR-34A;

D O I：

10.1159/000374024

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background: The anti-tumor effects of quercetin have been reported, but the underlying molecular mechanisms remain to be elucidated. The aim of present study was to explore the role of miRNA in the anticancer effects of quercetin. Methods: The differential miRNAs expression between the HepG2 and Huh7 cells treated by quercetin were detected by microarray. The xCELLigence, Flow cytometry, RT-PCR and Western blot were used to analyze the cell proliferation, cell apoptosis, cell cycle arrest, anti-tumor genes, and protein expression. Results: miR-34a was up-regulated in HepG2 cells treated by quercetin-exhibiting wild-type p53. When inhibiting the miR-34a, the sensitivity of the cells to quercetin decreased and the expression of the SIRT1 was up-regulated, but the acetylation of p53 and the expression of some genes related to p53 down-regulated. Conclusion: miR-34a plays an important role in the anti-tumor effects of querctin in HCC, miR-34a may be a tiemolecule between the p53 and SIRT1 and is composed of a p53/miR-34a/SIRT1 signal feedback loop, which could enhance apoptosis signal and significantly promote cell apoptosis. Copyright (C) 201 S. Karger Ag, Basel

引用

页码：2192 / 2202

页数：11

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