The stimulation of osteogenic differentiation of human adipose-derived stem cells by ionic products from akermanite dissolution via activation of the ERK pathway

被引：155

作者：

Gu, Huijie ^{[1
]}

Guo, Fangfang ^{[1
]}

Zhou, Xiao ^{[3
]}

Gong, Lunli ^{[1
]}

Zhang, Yun ^{[1
]}

Zhai, Wanyin ^{[2
]}

Chen, Lei ^{[2
]}

Cen, Lian ^{[1
]}

Yin, Shuo ^{[1
]}

Chang, Jiang ^{[2
]}

Cui, Lei ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Dept Plast & Reconstruct Surg, Shanghai Peoples Hosp 9, Shanghai 200011, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Ceram, Shanghai 200050, Peoples R China

[3] Hunan Prov Tumor Hosp, Dept Plast Surg, Changsha 410013, Hunan, Peoples R China

来源：

BIOMATERIALS | 2011年 / 32卷 / 29期

关键词：

Akermanite; Adipose-derived stem cells; Ions; Osteogenesis; Bone tissue engineering; MARK; BIOACTIVE GLASS DISSOLUTION; CALCIUM-SENSING RECEPTOR; IN-VITRO; PROLIFERATION; TISSUE; OSTEOBLASTS; KINASE; MINERALIZATION; BIOCERAMICS; EXPRESSION;

D O I：

10.1016/j.biomaterials.2011.06.003

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Our previous study indicates that akermanite, a type of Ca-, Mg-, Si-containing bioceramic, can promote the osteogenic differentiation of hASCs. To elucidate the underlying mechanism, we investigated the effect of the extract from akermanite, on proliferation and osteogenic differentiation of hASCs. The original extract was obtained at 200 mg akermanite/ml LG-DMEM and further diluted with LG-DMEM. The final extracts were denoted as 1/2, 1/4, 1/8, 1/16, and 1/32 extracts based on the concentrations of the original extract. The LDH assay and live/dead stain were used to reveal the cytotoxicity of the different extracts on hASCs, while the DNA assay was carried out to quantitatively evaluate the proliferation of cells after being cultured with the extracts for 1, 3 and 7 days. Flow cytometry for cell cycle analysis was carried out on cells cultured in two media (GM and 1/2 extract) in order to further analyze the effect of the extract on cell proliferation behaviors. Osteogenic differentiation of hASCs cultured in the extracts was detected by ALP expression and calcium deposition, and further confirmed by real-time PCR analysis. It was shown that Ca. Mg and Si ions in the extract could suppress the LDH release and proliferation of hASCs, whereas promote their osteogenic differentiation. Such effects were concentration-dependent with the 1/4 extract (Ca 2.36 mM, Mg 1.11 mM, Si 1.03 mM) being the optimum in promoting the osteogenic differentiation of hASCs. An immediate increase in ERK was observed in cells cultured in the 1/4 extract and such osteogenic differentiation of hASCs promoted by released ions could be blocked by MEK1-specific inhibitor, PD98059. Briefly, Ca, Mg and Si ions extracted from akermanite in the concentrations of 2.36, 1.11, 1.03 mM, respectively, could facilitate the osteogenic differentiation of hASCs via an ERK pathway, and suppress the proliferation of hASCs without significant cytotoxicity. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：7023 / 7033

页数：11

共 37 条

[1]

[Anonymous], 109935 ISOEN

[2] Divalent cations and ligands induce conformational changes that are highly divergent among β1 integrins [J].