Reversed-phase HPLC of peptides with tetraphenylporphyrin-based stationary phase and potentiometric detection with a copper electrode

被引：0

作者：

Trojanowicz, M

Martin, GB

Meyerhoff, ME

机构：

[1] SELECT TECHNOL INC,ANN ARBOR,MI 48103

[2] UNIV MICHIGAN,DEPT CHEM,ANN ARBOR,MI 48109

来源：

CHEMIA ANALITYCZNA | 1996年 / 41卷 / 04期

关键词：

HPLC; porphyrin-based stationary phase; peptides; potentiometric detection; metallic copper electrode;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The retention of selected amino acids and simple peptides has been investigated using columns with stationary phases with immobilized [5-(p-carboxyphenyl)-10,15,20-tri-phenyl]porphyrin and zinc(II) ion as coordination center. The use of potentiometry with a metallic copper electrode provides an especially sensitive detection of peptides containing histidine. The retention mechanism includes both interactions between the aromatic moieties of the solute molecule and immobilized metalloporphyrin and a complexation interaction between solutes and the metallic center of the metalloporphyrin.

引用

页码：521 / 530

页数：10

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