Metabolic Engineering Design Strategies for Increasing Acetyl-CoA Flux

被引：42

作者：

Ku, Jason T. ^{[1
]}

Chen, Arvin Y. ^{[1
]}

Lan, Ethan I. ^{[2
]}

机构：

[1] Natl Chiao Tung Univ, Inst Mol Med & Bioengn, Hsinchu 300, Taiwan

[2] Natl Chiao Tung Univ, Dept Biol Sci & Technol, Hsinchu 300, Taiwan

来源：

METABOLITES | 2020年 / 10卷 / 04期

关键词：

acetyl-CoA; metabolic bypass; pyruvate dehydrogenase; coenzyme A; PYRUVATE-DEHYDROGENASE COMPLEX; ESCHERICHIA-COLI STRAIN; COENZYME-A; PANTOTHENATE KINASE; POLY(3-HYDROXYBUTYRATE) PRODUCTION; PHOSPHOENOLPYRUVATE CARBOXYLASE; INTRACELLULAR CONCENTRATION; PHOTOSYNTHETIC PRODUCTION; ISOAMYL ACETATE; GENE-EXPRESSION;

D O I：

10.3390/metabo10040166

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetyl-CoA is a key metabolite precursor for the biosynthesis of lipids, polyketides, isoprenoids, amino acids, and numerous other bioproducts which are used in various industries. Metabolic engineering efforts aim to increase carbon flux towards acetyl-CoA in order to achieve higher productivities of its downstream products. In this review, we summarize the strategies that have been implemented for increasing acetyl-CoA flux and concentration, and discuss their effects. Furthermore, recent works have developed synthetic acetyl-CoA biosynthesis routes that achieve higher stoichiometric yield of acetyl-CoA from glycolytic substrates.

引用

页数：17

共 50 条

[41] Acute metabolic amplification of insulin secretion in mouse islets: Role of cytosolic acetyl-CoA
Panten, Uwe
Frueh, Eike
Reckers, Kirstin
Rustenbeck, Ingo
METABOLISM-CLINICAL AND EXPERIMENTAL, 2016, 65 (09): : 1225 - 1229
[42] Engineering acetyl-CoA metabolism to enhance stress tolerance of yeast by regulating membrane functionality
Wang, Dingkang
He, Zixi
Xia, Huan
Huang, Jun
Jin, Yao
Zhou, Rongqing
Hao, Liying
Wu, Chongde
FOOD MICROBIOLOGY, 2023, 115
[43] Molecular biology of cytosolic acetyl-CoA generation
Fatland, B
Anderson, M
Nikolau, BJ
Wurtele, ES
BIOCHEMICAL SOCIETY TRANSACTIONS, 2000, 28 : 593 - 595
[44] Valproyl-CoA and esterified valproic acid are not found in brains of rats treated with valproic acid, but the brain concentrations of CoA and acetyl-CoA are altered
Deutsch, J
Rapoport, SI
Rosenberger, TA
NEUROCHEMICAL RESEARCH, 2003, 28 (06) : 861 - 866
[45] Valproyl-CoA and Esterified Valproic Acid Are Not Found in Brains of Rats Treated with Valproic Acid, but the Brain Concentrations of CoA and Acetyl-CoA Are Altered
Joseph Deutsch
Stanley I. Rapoport
Thad A. Rosenberger
Neurochemical Research, 2003, 28 : 861 - 866
[46] Synthetic metabolic bypass for a metabolic toggle switch enhances acetyl-CoA supply for isopropanol production by Escherichia coli
Soma, Yuki
Yamaji, Taiki
Matsuda, Fumio
Hanai, Taizo
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2017, 123 (05) : 625 - 633
[47] METHODS FOR ANALYSIS OF ACETYL-COA SYNTHASE: APPLICATIONS TO BACTERIAL AND ARCHAEAL SYSTEMS
Grahame, David A.
METHODS IN ENZYMOLOGY: METHODS IN METHANE METABOLISM, PT A, 2011, 494 : 189 - 217
[48] Acetyl-CoA deficit in brain mitochondria in experimental thiamine deficiency encephalopathy
Jankowska-Kulawy, Agnieszka
Bielarczyk, Hanna
Pawelczyk, Tadeusz
Wroblewska, Malgorzata
Szutowicz, Andrzej
NEUROCHEMISTRY INTERNATIONAL, 2010, 57 (07) : 851 - 856
[49] Overexpression of acetyl-CoA carboxylase in Aspergillus terreus to increase lovastatin production
Hasan, Hanan
Abd Rahim, Muhammad Hafiz
Campbell, Leona
Carter, Dee
Abbas, Ali
Montoya, Alejandro
NEW BIOTECHNOLOGY, 2018, 44 : 64 - 71
[50] Hydrolysis of the acetyl-CoA allosteric activator by Staphylococcus aureus pyruvate carboxylase
Laseke, Amanda J.
Lohman, Jeremy R.
Maurice, Martin St.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2025, 764

← 1 2 3 4 5 →