Metabolic Engineering Design Strategies for Increasing Acetyl-CoA Flux

被引：42
|
作者：
Ku, Jason T. [1 ]
Chen, Arvin Y. [1 ]
Lan, Ethan I. [2 ]
机构：
[1] Natl Chiao Tung Univ, Inst Mol Med & Bioengn, Hsinchu 300, Taiwan
[2] Natl Chiao Tung Univ, Dept Biol Sci & Technol, Hsinchu 300, Taiwan
来源：
METABOLITES | 2020年 / 10卷 / 04期
关键词：
acetyl-CoA; metabolic bypass; pyruvate dehydrogenase; coenzyme A; PYRUVATE-DEHYDROGENASE COMPLEX; ESCHERICHIA-COLI STRAIN; COENZYME-A; PANTOTHENATE KINASE; POLY(3-HYDROXYBUTYRATE) PRODUCTION; PHOSPHOENOLPYRUVATE CARBOXYLASE; INTRACELLULAR CONCENTRATION; PHOTOSYNTHETIC PRODUCTION; ISOAMYL ACETATE; GENE-EXPRESSION;
D O I：
10.3390/metabo10040166
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Acetyl-CoA is a key metabolite precursor for the biosynthesis of lipids, polyketides, isoprenoids, amino acids, and numerous other bioproducts which are used in various industries. Metabolic engineering efforts aim to increase carbon flux towards acetyl-CoA in order to achieve higher productivities of its downstream products. In this review, we summarize the strategies that have been implemented for increasing acetyl-CoA flux and concentration, and discuss their effects. Furthermore, recent works have developed synthetic acetyl-CoA biosynthesis routes that achieve higher stoichiometric yield of acetyl-CoA from glycolytic substrates.
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页数：17
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