Quercetin inhibits transforming growth factor β1-induced epithelial-mesenchymal transition in human retinal pigment epithelial cells via the Smad pathway

被引：23

作者：

Cai, Wenting ^{[1
]}

Yu, Donghui ^{[1
]}

Fan, Jiaqi ^{[2
]}

Liang, Xiuwei ^{[3
]}

Jin, Huizi ^{[1
]}

Liu, Chang ^{[2
]}

Zhu, Meijiang ^{[1
]}

Shen, Tianyi ^{[1
]}

Zhang, Ruiling ^{[1
]}

Hu, Weinan ^{[4
]}

Wei, Qingquan ^{[1
]}

Yu, Jing ^{[1
,5
]}

机构：

[1] Tongji Univ, Shanghai Peoples Hosp 10, Dept Ophthalmol, 301 Middle Yanchang Rd, Shanghai 200072, Peoples R China

[2] Nanjing Med Univ, Dept Ophthalmol, Nanjing, Jiangsu, Peoples R China

[3] Nanchang Univ, Dept Ophthalmol, Nanchang, Jiangxi, Peoples R China

[4] Anhui Univ Sci & Technol, Dept Ophthalmol, Huainan, Peoples R China

[5] Ninghai First Hosp, Dept Ophthalmol, Ninghai, Zhejiang, Peoples R China

来源：

DRUG DESIGN DEVELOPMENT AND THERAPY | 2018年 / 12卷

基金：

中国国家自然科学基金;

关键词：

proliferative vitreoretinopathy; quercetin; epithelial-mesenchymal transition; transforming growth factor-beta 1; PROLIFERATIVE VITREORETINOPATHY; IN-VIVO; RESVERATROL; MIGRATION; FIBROSIS; INVASIVENESS; ANGIOGENESIS; SUPPRESSION; BRADYKININ; CYTOKINES;

D O I：

10.2147/DDDT.S185618

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Purpose: The purpose of this study was to evaluate the effect and mechanism of quercetin on TGF-beta 1-induced retinal pigment epithelial (RPE) cell proliferation, migration, and extracellular matrix secretion. Materials and methods: Cell counting kit-8, transwell, wound-healing assays, and ELISA were used to assess viability, migration, and collagen I secretion, respectively. Western blot analysis and qPCR were employed to detect mRNA and protein expression levels, respectively. Results: Quercetin suppressed TGF-beta 1-induced cell proliferation, migration, and collagen I secretion. The results also showed that mRNA and protein expression of epithelial-mesenchymal transition (EMT)-related markers such as alpha-smooth muscle actin and N-cadherin was downregulated by quercetin in TGF-beta 1-treated RPE cells; conversely, quercetin upregulated the expression of E-cadherin and tight junction protein 1 (ZO-1). In addition, quercetin could inhibit mRNA and protein expression of matrix metalloproteinases. Quercetin may reverse the progression of EMT via the Smad2/3 pathway. Conclusion: Our results demonstrate the protective effects of quercetin on RPE cell EMT, revealing a potential therapeutic agent for proliferative vitreoretinopathy treatment.

引用

页码：4149 / 4161

页数：13

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