LncRNA ZEB1-AS1 predicts unfavorable prognosis in gastric cancer

被引：56
作者：
Li, Yanliang [1 ,2 ]
Wen, Xiaowen [1 ]
Wang, Longgang [1 ]
Sun, Xianjun [1 ]
Ma, Heng [1 ]
Fu, Zheng [3 ]
Li, Leping [2 ]
机构：
[1] Shandong Univ, Dept Gen Surg, Shandong Canc Hosp, Shandong Acad Med Sci, 440 Jiyan Rd, Jinan 250117, Shandong, Peoples R China
[2] Shandong Univ, Dept Gen Surg, Shandong Prov Hosp, 324 Jingwuweiqi Rd, Jinan 250021, Shandong, Peoples R China
[3] Shandong Univ, Shandong Canc Hosp, Dept Radiol, Shandong Acad Med Sci, 440 Jiyan Rd, Jinan 250117, Shandong, Peoples R China
来源：
SURGICAL ONCOLOGY-OXFORD | 2017年 / 26卷 / 04期
关键词：
LncRNA; ZEB1-AS1; ZEB1; EMT; Metastasis; Gastric cancer; Prognosis; LONG NONCODING RNA; EPITHELIAL-MESENCHYMAL TRANSITION; ABERRANT EXPRESSION; METASTASIS; STATISTICS; MIGRATION; ANRIL; PVT1;
D O I：
10.1016/j.suronc.2017.09.008
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Objectives: LncRNA ZEB1 Antisense 1 (ZEB1-AS1) has been suggested to be an oncogenic role in human hepatocellular carcinoma, osteosarcoma, glioma and esophageal carcinoma progression. However, the clinical significance and biological function of ZEB1-AS1 in gastric cancer is poorly understood. Methods: Levels of ZEB1-AS1 expression in gastric cancer tissues and cell lines were detected by qRT-PCR. Loss-of-function and gain-of-function studies were conducted to explore the biological function of ZEB1-AS1 in gastric cancer cells migration, invasion and EMT process. Rescued-function studies were performed to explore the association between ZEB1-AS1 and ZEB1 in gastric cancer cells migration, invasion and EMT process. Results: ZEB1-AS1 was overexpressed in gastric cancer tissues and cell lines, and correlated with malignant status and prognosis in gastric cancer patients. ZEB1-AS1 regulated gastric cancer cells migration, invasion and EMT process. ZEB1-AS1 positively regulated ZEB1 expression in gastric cancer cells, and had a strongly positive correlation with ZEB1 expression in gastric cancer tissues. Rescued-function studies showed ZEB1 was critical for ZEB1-AS1 induced gastric cancer cells migration, invasion and EMT process. Conclusions: ZEB1-AS1 served as oncogenic roles in the regulation of gastric cancer cells migration, invasion and EMT process through modulating ZEB1. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：527 / 534
页数：8
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