Alterations in lipid transfers to HDL associated with the presence of coronary artery disease in patients with type 2 diabetes mellitus

被引:21
作者
Sprandel, Marilia C. O. [1 ]
Hueb, Whady A. [2 ]
Segre, Alexandre [2 ]
Ramires, Jose A. F. [2 ]
Kalil-Filho, Roberto [2 ]
Maranhao, Raul C. [1 ,3 ]
机构
[1] Univ Sao Paulo, Med Sch Hosp, Inst Heart, Lipid Metab Lab, Sao Paulo, Brazil
[2] Univ Sao Paulo, Inst Heart, Med Sch Hosp, Clin Cardiol Div, Sao Paulo, Brazil
[3] Univ Sao Paulo, Fac Pharmaceut Sci, Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
Coronary artery disease; Type; 2; diabetes; Lipid transfers; High-density lipoprotein; HDL; Nanoparticles; Cholesterol; HIGH-DENSITY-LIPOPROTEIN; CHOLESTERYL ESTER TRANSFER; TRANSFER PROTEIN-ACTIVITY; HEART-DISEASE; SERUM-LIPIDS; PLASMA; EFFLUX; ATHEROSCLEROSIS; DYSLIPIDEMIA; PREDICTORS;
D O I
10.1186/s12933-015-0270-8
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: We previously showed that unesterified-cholesterol transfer to high-density lipoprotein ( HDL), a crucial step in cholesterol esterification and role in reverse cholesterol transport, was diminished in non-diabetic patients with coronary artery disease ( CAD). The aim was to investigate whether, in patients with type 2 diabetes mellitus ( T2DM), the occurrence of CAD was also associated with alterations in lipid transfers and other parameters of plasma lipid metabolism. Methods: Seventy-nine T2DM with CAD and 76 T2DM without CAD, confirmed by cineangiography, paired for sex, age ( 40-80 years), BMI and without statin use, were studied. In vitro transfer of four lipids to HDL was performed by incubating plasma of each patient with a donor emulsion containing radioactive lipids during 1 h at 37 degrees C. Lipids transferred to HDL were measured after chemical precipitation of non-HDL fractions and the emulsion. Results are expressed as % of total radioactivity of each lipid in HDL. Results: In T2DM + CAD, LDL-cholesterol and apo B were higher than in T2DM. T2DM + CAD also showed diminished transfer to HDL of unesterified cholesterol ( T2DM + CAD = 7.6 +/- 1.2; T2DM = 8.2 +/- 1.5 %, p < 0.01) and of cholesteryl-esters ( 4.0 +/- 0.6 vs 4.3 +/- 0.7, p < 0.01). Unesterified cholesterol in the non-HDL serum fraction was higher in T2DM + CAD ( 0.93 +/- 0.20 vs 0.85 +/- 0.15, p = 0.02) and CETP concentration was diminished ( 2.1 +/- 1.0 vs 2.5 +/- 1.1, p = 0.02). Lecithin-cholesterol acyltransferase activity, HDL size and lipid composition were equal. Conclusion: Reduction in T2DM + CAD of cholesterol transfer to HDL may impair cholesterol esterification and reverse cholesterol transport and altogether with simultaneous increased plasma unesterified cholesterol may facilitate CAD development in T2DM.
引用
收藏
页数:9
相关论文
共 37 条
[1]   Phospholipid transfer protein activity and incident type 2 diabetes mellitus [J].
Abbasi, Ali ;
Dallinga-Thie, Geesje M. ;
Dullaart, Robin P. F. .
CLINICA CHIMICA ACTA, 2015, 439 :38-41
[2]   Low Levels of High-Density Lipoprotein Cholesterol and Increased Risk of Cardiovascular Events in Stable Ischemic Heart Disease Patients A Post-Hoc Analysis From the COURAGE Trial (Clinical Outcomes Utilizing Revascularization and Aggressive Drug Evaluation) [J].
Acharjee, Subroto ;
Boden, William E. ;
Hartigan, Pamela M. ;
Teo, Koon K. ;
Maron, David J. ;
Sedlis, Steven P. ;
Kostuk, William ;
Spertus, John A. ;
Dada, Marcin ;
Chaitman, Bernard R. ;
Mancini, G. B. John ;
Weintraub, William S. .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2013, 62 (20) :1826-1833
[3]   Cholesteryl ester transfer protein inhibition as a strategy to reduce cardiovascular risk [J].
Barter, Philip J. ;
Rye, Kerry-Anne .
JOURNAL OF LIPID RESEARCH, 2012, 53 (09) :1755-1766
[4]   LIPID NOMENCLATURE - RECOMMENDATIONS REGARDING THE REPORTING OF SERUM LIPIDS AND LIPOPROTEINS MADE BY THE COMMITTEE ON LIPID AND LIPOPROTEIN NOMENCLATURE OF THE AMERICAN SOCIETY-FOR-THE-STUDY-OF-ARTERIOSCLEROSIS [J].
BRAGDON, JH ;
EDER, HA ;
GOULD, RG ;
HAVEL, RJ .
CIRCULATION RESEARCH, 1956, 4 (02) :129-129
[5]   Deposition of free cholesterol in the blood vessels of patients with coronary artery disease: A possible novel mechanism for atherogenesis [J].
Couto, Ricardo D. ;
Dallan, Luis A. O. ;
Lisboa, Luiz A. F. ;
Mesquita, Carlos H. ;
Vinagre, Carmen G. C. ;
Maranhao, Raul C. .
LIPIDS, 2007, 42 (05) :411-418
[6]   Plasma cholesteryl ester transfer protein mass and phospholipid transfer protein activity are associated with leptin in type 2 diabetes mellitus [J].
Dullaart, R. P. F. ;
de Vries, R. ;
Dallinga-Thie, G. M. ;
van Tol, A. ;
Sluiter, W. J. .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2007, 1771 (01) :113-118
[7]  
FRIEDEWALD WT, 1972, CLIN CHEM, V18, P499
[8]   Fractional esterification rate of cholesterol and ratio of triglycerides to HDL-cholesterol are powerful predictors of positive findings on coronary angiography [J].
Frohlich, J ;
Dobiásová, M .
CLINICAL CHEMISTRY, 2003, 49 (11) :1873-1880
[9]   Selective peroxisome proliferator-activated receptora modulators (SPPARMα): The next generation of peroxisome proliferator-activated receptor α-agonists [J].
Fruchart, Jean-Charles .
CARDIOVASCULAR DIABETOLOGY, 2013, 12
[10]   HIGH-DENSITY LIPOPROTEIN CHOLESTEROL AND CARDIOVASCULAR-DISEASE - 4 PROSPECTIVE AMERICAN-STUDIES [J].
GORDON, DJ ;
PROBSTFIELD, JL ;
GARRISON, RJ ;
NEATON, JD ;
CASTELLI, WP ;
KNOKE, JD ;
JACOBS, DR ;
BANGDIWALA, S ;
TYROLER, HA .
CIRCULATION, 1989, 79 (01) :8-15