De novo transcriptome sequencing of Agropyron cristatum to identify available gene resources for the enhancement of wheat

被引：48

作者：

Zhang, Jinpeng ^{[1
]}

Liu, Weihua ^{[1
]}

Han, Haiming ^{[1
]}

Song, Liqiang ^{[1
]}

Bai, Li ^{[1
]}

Gao, Zhihui ^{[1
]}

Zhang, Yan ^{[1
]}

Yang, Xinming ^{[1
]}

Li, Xiuquan ^{[1
]}

Gao, Ainong ^{[1
]}

Li, Lihui ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

来源：

GENOMICS | 2015年 / 106卷 / 02期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

Transcriptomes; P genome; Disease resistance; Genetic diversity; Wild relative; Agropyron cristatum; INTERGENIC NONCODING RNAS; CRESTED WHEATGRASS; WATER-DEFICIT; DRAFT GENOME; RESISTANCE; EXPRESSION; STRESS; TOLERANCE; CLONING; DOMAIN;

D O I：

10.1016/j.ygeno.2015.04.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Agropyron cristatum is a wild grass of the tribe Triticeae that is widely grown in harsh environments. As a wild relative of wheat, A. cristatum carries many resistance genes that could be used to broaden the genetic diversity of wheat Here, we report the transcriptome sequencing of the flag leaf and young spike tissues of a representative tetraploid A. cristatum. More than 90 million reads from the two tissues were assembled into 73,664 unigenes. All unigenes were functionally annotated against the KEGG, COG, and Gene Ontology databases and predicted long non-coding RNAs. Pfam prediction demonstrates that A. cristatum carries an abundance of stress resistance genes. The extent of specific genes and rare alleles make A. cristatum a vital genetic reservoir for the improvement of wheat. Altogether, the available gene resources in A. cristatum facilitate efforts to harness the genetic diversity of wild relatives to enhance wheat. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：129 / 136

页数：8

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