Simple fabrication of polymer-based Trametes versicolor laccase for decolorization of malachite green

被引:17
作者
Zhang, Xiaolin [1 ]
Zhang, Shujuan [1 ]
Pan, Bingcai [1 ]
Hua, Ming [1 ]
Zhao, Xin [1 ]
机构
[1] Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210093, Peoples R China
关键词
Hybrid biocatalyst; Anion exchanger; Dye decoloration; Stability; Reuse; IMMOBILIZATION; ENZYME; DEGRADATION; DYES; ENCAPSULATION; CONFINEMENT; OXIDATION; SUPPORTS; LIGNIN; BEADS;
D O I
10.1016/j.biortech.2011.11.063
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
A highly efficient and stable biocatalyst (denoted D201_Lac) was fabricated by encapsulating Trametes versicolor laccase within a macroporous and strongly basic exchange resin D201 through a simple adsorption process. Transmission electron micrographs and Fourier transform infrared spectra of the resultant D201_Lac proved that nanosized laccase clusters were embedded into the inner nano-pores/channels of 0201. As compared to the free laccase, D201_Lac showed enhanced resistance in the pH range of 3-7 or at temperature of 30-60 degrees C. Besides, negligible laccase was leached out from the host polymer D201 in solution of pH 3-7 and NaCl concentration up to 0.5 M, which might be attributed to the electrostatic attraction and the possible twining between long-chain laccase and the cross-linking host resin. Continuous seven-cycle batch decoloration of malachite green demonstrates that decoloration efficiency of D201_Lac kept constant for more than 320-h operation. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:16 / 20
页数:5
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