Design, Synthesis, and Characterization of Nucleic-Acid-Functionalized Gold Surfaces for Biomarker Detection

被引：30

作者：

Adams, Nicholas M. ^{[1
,2
]}

Jackson, Stephen R. ^{[1
]}

Haselton, Frederick R. ^{[2
]}

Wright, David W. ^{[1
]}

机构：

[1] Vanderbilt Univ, Dept Chem, VU Stn B 351822, Nashville, TN 37235 USA

[2] Vanderbilt Univ, Dept Biomed Engn, VU Stn B 351631, Nashville, TN 37235 USA

来源：

LANGMUIR | 2012年 / 28卷 / 02期

基金：

比尔及梅琳达.盖茨基金会;

关键词：

SEQUENCE-SPECIFIC DETECTION; BIO-BARCODE ASSAY; IMMUNO-PCR; ELECTROCHEMICAL DETECTION; HYBRIDIZATION EFFICIENCY; ULTRASENSITIVE DETECTION; MOLECULAR BEACONS; ENERGY-TRANSFER; PARTICLE-SIZE; DNA;

D O I：

10.1021/la2028862

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nucleic-acid-functionalized gold surfaces have been used extensively for he development of biological sensors. The development of an effective biomarker detection assay requires careful design, synthesis, and characterization of probe components, in this Feature Article, we describe fundamental probe development constraints and provide a critical appraisal of the current methodologies and applications in the field. We discuss critical issues and obstacles that impede the sensitivity and reliability of the sensors to underscore the challenges that must be met to advance the field of biomarker detection.

引用

页码：1068 / 1082

页数：15

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