Characterization of the expression profiles of calpastatin (CAST) gene in chicken

被引:15
作者
Zhang, Zeng-Rong [1 ,2 ]
Jiang, Xiao-Song [2 ]
Du, Hua-Rui [2 ]
Zhu, Qing [1 ]
Li, Xiao-Cheng [3 ]
Yang, Chao-Wu [2 ]
Liu, Yi-Ping [1 ]
机构
[1] Sichuan Agr Univ, Coll Anim Sci & Technol, Yaan 625014, Sichuan, Peoples R China
[2] Sichuan Anim Sci Acad, Chengdu 610066, Hunan, Peoples R China
[3] Sichuan Dahen Poultry Breeding Co, Chengdu 610066, Sichuan, Peoples R China
关键词
Chicken; CAST; Real-time PCR; Age; Tissue; MUSCLE PROTEIN-TURNOVER; SKELETAL-MUSCLE; PROTEOLYTIC SYSTEM; MEAT TENDERNESS; BREAST MUSCLE; GROWTH; DEGRADATION; CALPAIN; QUALITY; BROILER;
D O I
10.1007/s11033-011-0926-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The calpain system, a Ca(2+)-activated protease family, plays an important role in postmortem tenderization of skeletal muscle due to its involvement in the degradation of important myofibrillar and associated proteins, as well as in cytoskeletal remodeling and regulation of muscle growth. In this study, we quantified the expression of calpastatin (CAST) in two Chinese chicken breeds (mountainous black-bone chicken breed (MB) and a commercial meat type chicken breed (S01)), to discern the tissue and age-related specific expression pattern and its potential role on muscle tissue metabolism. Real-time quantitative PCR (RT-qPCR) assay was developed for accurate measurement of CAST mRNA levels in various tissues from chicken with different ages (0, 2, 4, 6, 8, 10, and 12 week). CAST mRNA was detected in collected organs. The heart and leg muscle tissues had the highest expression of CAST than other tissues from the same chicken (P < 0.01). Age-related expression pattern of CAST gene was evident in breast muscle, liver, and brain tissues (P < 0.05), but not in heart and leg muscle tissues (P > 0.05). Overall, the CAST mRNA level exhibited a "rise-decline-rise-decline" developmental change in breast muscle and liver, with the highest expression at 2 weeks and the lowest expression at 8 weeks. The S01 chicken had significantly higher expression of CAST in breast muscle and heart than the MB chicken (P < 0.05) at 10 weeks. Our results suggested the CAST expression may be related to muscle fiber development.
引用
收藏
页码:1839 / 1843
页数:5
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