Agaricus bisporus-derived β-glucan prevents obesity through PPAR γ downregulation and autophagy induction in zebrafish fed by chicken egg yolk

被引：24

作者：

Li, Xiumin ^{[1
]}

Xue, Yu ^{[1
]}

Pang, Liang ^{[2
]}

Len, Bo ^{[1
]}

Lin, Zhichao ^{[1
]}

Huang, Jiafu ^{[1
]}

ShangGuan, Zhaoshui ^{[3
]}

Pan, Yutian ^{[1
]}

机构：

[1] Minnan Normal Univ, Engn Technol Ctr Mushroom Ind, 36 Xianqianzhi Rd, Zhangzhou 363000, Fujian, Peoples R China

[2] Minnan Normal Univ, Coll Phys Educ, Zhangzhou 363000, Fujian, Peoples R China

[3] Xiamen Univ, Affiliated Hosp 1, Cent Lab, Xiamen 361003, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2019年 / 125卷

关键词：

beta-Glucan; PPAR gamma; Lipid metabolism; Autophagy; Zebrafish; PROLIFERATOR-ACTIVATED RECEPTORS; TRIGLYCERIDE TRANSFER PROTEIN; LIPID-METABOLISM; EXPRESSION; FAT; INFLAMMATION; GENE;

D O I：

10.1016/j.ijbiomac.2018.12.122

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-(1,4)-D-Glucan with (1,2) and (1,6)-linked branches (short for beta-glucan), extracted from Agaricus bisporus (Lange) Sing, had significant anti-obesity and lowering-fat effect. FITC-beta-glucan was absorbed by adipocytes of zebrafish larvae when stained by Nile Red. P-Glucan decreased the adiposity mass, reduced the expression of ppar g, mtp, L-fabp, ifabp in ISH, which was coincident as the results of RT-PCT. beta-Glucan lowered the level of C/EBP alpha, c SREBP1, LXR alpha, PPAR gamma by WB analysis, which were accompanied by an increase level in LC3 II/LC3 I and a decline level in p62 in dose-dependent manner. This study explored the effect and mechanisms of Agaricus bisporus derived-beta-glucan to regulate lipid metabolism and prevent lipid deposits, and provided the experimental data for its use in diet food and food addictive. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：820 / 828

页数：9

共 47 条

[1] Zebrafish Lipid Metabolism: From Mediating Early Patterning to the Metabolism of Dietary Fat and Cholesterol [J].

Anderson, Jennifer L. ;

Carten, Juliana D. ;

Farber, Steven A. .

ZEBRAFISH: CELLULAR AND DEVELOPMENTAL BIOLOGY, PT B, THIRD EDITION, 2011, 101 :111-141

[2]

[Anonymous], 2012, EURO SURVEILL, V17

[3] Chemistry, physico-chemistry and applications linked to biological activities of β-glucans [J].

Barsanti, Laura ;

Passarelli, Vincenzo ;

Evangelista, Valtere ;

Frassanito, Anna Maria ;

Gualtieri, Paolo .

NATURAL PRODUCT REPORTS, 2011, 28 (03) :457-466

[4] Clinical and Physiological Perspectives of -Glucans: The Past, Present, and Future [J].

Bashir, Khawaja Muhammad Imran ;

Choi, Jae-Suk .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2017, 18 (09)

[5] Pathways commonly dysregulated in mouse and human obese adipose tissue: FAT/CD36 modulates differentiation and lipogenesis [J].

Berger, E. ;

Heraud, S. ;

Mojallal, A. ;

Lequeux, C. ;

Weiss-Gayet, M. ;

Damour, O. ;

Geloen, A. .

ADIPOCYTE, 2015, 4 (03) :161-180

[6] Weighing in on Adipocyte Precursors [J].

Berry, Ryan ;

Jeffery, Elise ;

Rodeheffer, Matthew S. .

CELL METABOLISM, 2014, 19 (01) :8-20

[7] Unexpected novel relational links uncovered by extensive developmental profiling of nuclear receptor expression [J].

Bertrand, Stephanie ;

Thisse, Bernard ;

Tavares, Raquel ;

Sachs, Laurent ;

Chaumot, Arnaud ;

Bardet, Pierre-Luc ;

Escriva, Hector ;

Duffraisse, Maryline ;

Marchand, Oriane ;

Safi, Rachid ;

Thisse, Christine ;

Laudet, Vincent .

PLOS GENETICS, 2007, 3 (11) :2085-2100

[8] Differential expression of peroxisome proliferator-activated receptors (PPARs): Tissue distribution of PPAR-alpha, -beta, and -gamma in the adult rat [J].

Braissant, O ;

Foufelle, F ;

Scotto, C ;

Dauca, M ;

Wahli, W .

ENDOCRINOLOGY, 1996, 137 (01) :354-366

[9]

Broeder M.J.D., 2015, PPAR RES, V2015

[10] Immune recognition -: A new receptor for β-glucans [J].

Brown, GD ;

Gordon, S .

NATURE, 2001, 413 (6851) :36-37

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