Mesona chinensis Benth polysaccharides protect against oxidative stress and immunosuppression in cyclophosphamide-treated mice via MAPKs signal transduction pathways

In this study, the immune responses of Mesona chinensis Benth polysaccharides (MP) in vitro and in vivo were investigated. Results showed that MP presented immunomodulatory activities on macrophages and T lymphocytes in vitro. Compared with the cyclophosphamide (Cy)-induced immunosuppressive mice, the body weights, spleen indexes (3.45 to 4.91) and thymus indexes (0.78 to 1.04) of the mice treated with MP were increased, and the peripheral blood levels were recovered. MP treatment also increased superoxide dismutase, glutathione peroxidase and catalase activities, and reduced malondialdehyde levels to enhance the total antioxidant capacity of Cytreated mice. In addition, MP significantly elevated IL-2, NO, and IFN-gamma secretions of splenic lymphocytes and spleen, while MP mainly exerts an immune effect by regulating T lymphocytes. Furthermore, MP possessed the immunomodulatory activity by up-regulating the phosphorylation levels of proteins factors (c-Jun N-terminal kinase, extracellular regulated protein kinase and p38 kinase) in mitogen activated protein kinases signaling pathways. This study suggested that MP may be explored as a natural immune stimulant for functional food and nutraceutical industries. (C) 2020 Elsevier B.V. All rights reserved.