Surface-enhanced Raman scattering for cancer diagnostics:: detection of the BClL2 gene

被引：25

作者：

Culha, M ^{[1
]}

Stokes, D ^{[1
]}

Vo-Dinh, T ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Adv Biomed Sci & Technol Grp, Oak Ridge, TN 37831 USA

来源：

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS | 2003年 / 3卷 / 05期

关键词：

BCL2; cancer; detection; immobilization; rhodamine-B; SAM; SERS;

D O I：

10.1586/14737159.3.5.669

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced Raman scattering-active platforms. A self-assembled monolayer system composed of mercapto hexane-labeled, single-strand DNA (SH-(CH2)(6)-ssDNA/6-mercapto-1-hexanol) is formed on a silver surface. The Raman-active dye-labeled gene is hybridized with its complementary probe, which is immobilized on the silver surface. The surface-enhanced Raman gene probes in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not involve the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The utility of this approach is investigated.

引用

页码：669 / 675

页数：7

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