Construction and characterization of an infectious cDNA clone of Echovirus 25

被引：9

作者：

Hou, Wangheng ^{[1
]}

Yang, Lisheng ^{[1
]}

Li, Shuxuan ^{[1
]}

Yu, Hai ^{[1
]}

Xu, Longfa ^{[1
]}

He, Delei ^{[1
]}

Chen, Mengyuan ^{[1
]}

He, Shuizhen ^{[2
]}

Ye, Xiangzhong ^{[3
]}

Que, Yugiong ^{[1
]}

Shih, James Wai Kuo ^{[1
]}

Cheng, Tong ^{[1
]}

Xia, Ningshao ^{[1
]}

机构：

[1] Xiamen Univ, Sch Life Sci, State Key Lab Mol Vaccinol & Mol Diagnost, Natl Inst Diagnost & Vaccine Dev Infect Dis, Xiamen 361102, Peoples R China

[2] Xiamen Ctr Dis Control & Prevent, Xiamen, Fujian, Peoples R China

[3] Beijing Wantai Biol Pharm Enterprise Co Ltd, Beijing 102206, Peoples R China

来源：

VIRUS RESEARCH | 2015年 / 205卷

基金：

中国国家自然科学基金;

关键词：

Echovirus; 25; Infectious cDNA clone; Sequence analyses; Rescued virus; ASEPTIC-MENINGITIS; OUTBREAK; SEQUENCE; ENTEROVIRUSES; AMPLIFICATION; DETERMINANTS; CHILDREN; ILLNESS; STRAIN; VIRUS;

D O I：

10.1016/j.virusres.2015.05.011

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Echovirus 25 (E-25) is a member of the enterovirus family and a common pathogen that induces hand, foot, and mouth disease (HFMD), meningitis, skin rash, and respiratory illnesses. In this study, we constructed and characterized an infectious full-length E-25 cDNA clone derived from the XM0297 strain, which was the first subgenotype D6 strain isolated in Xiamen, China. The 5'-Untranslated Regions (5'-UTR), P3 (3A-3B, 3D) and P3 (3C) regions of this E-25 (XM0297) strain were highly similar to EV-B77, E-16 and E-13, respectively. Our data demonstrate that the rescued E-25 viruses exhibited similar growth kinetics to the prototype virus strain XM0297. We observed the rescued viral particles using transmission electron microscope (TEM) and found them to possess an icosahedral structure, with a diameter of approximately 30 nm. The cross neutralization test demonstrated that the E-25 (XM0297) strain immune serum could not neutralize EV-A71, CV-A16 or CV-B3; likewise, the EV-A71 and CV-A16 immune serum could not neutralize E-25 (XM0297). The availability of this infectious clone will greatly enhance future virological investigations and possible vaccine development against E-25. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：41 / 44

页数：4

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