Uptake of cell-penetrating peptides in yeasts

被引：36

作者：

Holm, T ^{[1
]}

Netzereab, S ^{[1
]}

Hansen, M ^{[1
]}

Langel, Ü ^{[1
]}

Hällbrink, M ^{[1
]}

机构：

[1] Univ Stockholm, Arrhenius Lab, Dept Neurochem & Neurotoxicol, S-10691 Stockholm, Sweden

来源：

FEBS LETTERS | 2005年 / 579卷 / 23期

关键词：

cell-penetrating peptides; internalization; degradation; Saccharomyces cerevisiae; Candida albicans;

D O I：

10.1016/j.febslet.2005.07.099

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The uptake of different cell-penetrating peptides (CPPs) in two yeast species, Saccharomyces cerevisiae and Candida albicans, was studied using fluorescence HPLC-analyses of cell content. Comparison of the ability of penetratin, pVEC and (KFF)(3)K to traverse the yeast cell envelope shows that the cellular uptake of the peptides varies widely. Moreover, the intracellular degradation of the CPPs studied varies from complete stability to complete degradation. We show that intracellular degradation into membrane impermeable products can significantly contribute to the fluorescence signal. pVEC displayed highest internalizing capacity, and considering its stability in both yeast species, it is an attractive candidate for further studies. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5217 / 5222

页数：6

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