PREVALENCE OF CLOSTRIDIUM DIFFICILE PCR RIBOTYPES IN BULGARIA, 2008-2010

被引:0
作者
Ivanova, Kate [1 ]
Petrov, Petar [1 ]
Asseva, Galina [1 ]
Dobreva, Elina [1 ]
Ivanov, Ivan [1 ]
Vatcheva-Dobrevska, Rositza [1 ]
Marina, Maya [1 ]
Kantardjiev, Todor [1 ]
Notermans, Daan [2 ]
Kuijper, Ed [3 ]
机构
[1] Natl Ctr Infect & Parasit Dis, Sofia 1504, Bulgaria
[2] Natl Inst Publ Hlth & Environm RIVM, Ctr Infect Dis Control Netherlands CIb, Bilthoven, Netherlands
[3] Leiden Univ, Dept Med Microbiol, Natl Reference Lab Clostridium Difficile, Leiden, Netherlands
来源
COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES | 2011年 / 64卷 / 07期
关键词
Clostridium difficile; diarrhoea; toxins; ribotypes; Bulgaria; TOXIN-B; NETHERLANDS; INFECTION; OUTBREAK; DISEASE; STRAIN; KOREA; PIGS;
D O I
暂无
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Clostridium difficile is one of the most important causative agents of severe diarrhoea in hospitalised patients treated with antibiotics. Since 2008, Bulgaria participated in the Pan-European Surveillance Study investigating the prevalence of C. difficile infections (CDI) in different European countries. In the period November 2008 - March 2010, the incidence of CDI in nine participating hospitals was 7.94 per 10 000 patient admissions (0.34 per 10 000 patient-days). In total, sixty five fecal samples from patients with mild to severe enterocolitis and previous antibiotic treatment were investigated for CDI. Strains were typed and further characterized for the presence of toxins A (TcdA), B (TcdB) and binary toxins (CdtA and CdtB). Of 65 stool samples included, 15 were toxin and culture positive for C. difficile. Six of the isolates (40%) belonged to PCR ribotype 017 (TcdA(-).; TcdB(+); CdtA/B(-)), followed by 002 (n = 2 isolates) and 014 (n = 2 isolates). The remaining C. difficile isolates were typed as 012 (n = 1), 046 (n = 1), 078 ribotypes (n = 1) and 2 isolates were untypable. In conclusion, after the Pan-European surveillance study, a laboratory-based surveillance of CDI has been introduced in Bulgaria. The most prevalent C. difficile ribotype in Bulgaria was 017 (40%). All determined PCR ribotypes were found to be associated with severe CDI and a high percentage of lethal outcomes, indicating that improvements of surveillance with appropriate clinical and epidemiological criteria is warranted.
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页码:1051 / 1058
页数:8
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