Purification of peanut proteins for further use in affinity chromatography and as immunogens

被引：1

作者：

Baumgartner, S ^{[1
]}

Hemetsberger, C ^{[1
]}

Drs, E ^{[1
]}

Pichler, H ^{[1
]}

Krska, R ^{[1
]}

机构：

[1] IFA Tulln, Ctr Analyt Chem, A-3430 Tulln, Austria

来源：

JOURNAL OF SEPARATION SCIENCE | 2003年 / 26卷 / 14期

关键词：

peanut proteins; chicken antibodies lgY; ion chromatography; affinity chromatography; immuno blot;

D O I：

10.1002/jssc.200301433

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Chickens were immunised with peanut protein extracts. Because of the high cross reactivity, the IgY antibodies were purified by means of an affinity column. An ion chromatographically purified peanut protein fraction was therefore coupled to the affinity support material and this column was used for further IgY purification.

引用

收藏

页码：1284 / 1286

页数：3

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