Rapid modification of capillaries by chicken egg white and their uses for separation of proteins in capillary isoelectric focusing

被引:0
作者
Yang, C [1 ]
Zhang, WB [1 ]
Zhang, YK [1 ]
机构
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
关键词
modification of capillary; chicken egg white; isoelectric focusing;
D O I
暂无
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple and rapid method. for modification of; capillaries with a solution of chicken egg white is developed. The modification avoids the sorption activity of proteins onto the capillary inner walls. The capillaries are stable in. mild conditions and are suitable in capillary isoelectric focusing (CIEF) for the separation in biological samples.
引用
收藏
页码:1097 / 1100
页数:4
相关论文
共 17 条
[1]   Cloud point extraction as a preconcentration step prior to capillary electrophoresis [J].
Carabias-Martínez, R ;
Rodríguez-Gonzalo, E ;
Domínguez-Alvarez, J ;
Hernández-Méndez, J .
ANALYTICAL CHEMISTRY, 1999, 71 (13) :2468-2474
[2]   Capillary electrophoretic separation of glycoproteins [J].
Chen, Y .
SCIENCE IN CHINA SERIES B-CHEMISTRY, 1998, 41 (01) :71-76
[3]   Characterization of poly(dimethylacrylamide) and the combination of poly(vinyl alcohol) and cetyltrimethylanlmonium bromide as dynamic electroosmotic flow suppression agents in capillary electrophoresis [J].
Chiari, M ;
Damin, F ;
Reijenga, JC .
JOURNAL OF CHROMATOGRAPHY A, 1998, 817 (1-2) :15-23
[4]  
Dougherty A M., 1997, Handbook o f Capillary Electrophoresis, VSecond, P675
[5]   A NEW-TYPE OF PH-STABLE AND DETERGENT-STABLE COATING FOR ELIMINATION OF ELECTROENDOOSMOSIS AND ADSORPTION IN (CAPILLARY) ELECTROPHORESIS [J].
HJERTEN, S ;
KUBO, K .
ELECTROPHORESIS, 1993, 14 (5-6) :390-395
[6]   Highly efficient protein separations in high-performance capillary electrophoresis, using hydrophilic coatings on polysiloxane-bonded columns [J].
Huang, MX ;
Mitchell, D ;
Bigelow, M .
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 677 (01) :77-84
[7]  
Kang Jingwu, 1998, CHINESE J CHROMATOGR, V16, P26
[8]   Automated particle electrophoresis: Modeling and control of adverse chamber surface properties [J].
Knox, RJ ;
Burns, NL ;
Van Alstine, JM ;
Harris, JM ;
Seaman, GVF .
ANALYTICAL CHEMISTRY, 1998, 70 (11) :2268-2279
[9]   Epoxy resin coatings for capillary zone electrophoretic separation of basic proteins [J].
Liu, Y ;
Fu, RN ;
Gu, JL .
JOURNAL OF CHROMATOGRAPHY A, 1996, 723 (01) :157-167
[10]  
Pacáková V, 2001, ELECTROPHORESIS, V22, P459, DOI 10.1002/1522-2683(200102)22:3<459::AID-ELPS459>3.0.CO