Cryosurvival and in vitro fertilizing capacity postthaw is improved when boar spermatozoa are frozen in the presence of seminal plasma from good freezer boars

被引:104
作者
Hernandez, Marta
Roca, Jordi
Calvete, Juan J.
Sanz, Libia
Muino-Blanco, Teresa
Cebrian-Perez, Jose A.
Vazquez, Juan M.
Martinez, Emilio A.
机构
[1] Univ Murcia, Fac Vet Med, Dept Med & Anim Surg, Murcia, Spain
[2] CSIC, Spanish Natl Res Council, Inst Biomed, Valencia, Spain
[3] Univ Zaragoza, Fac Med Vet, Dept Biochem & Mol & Cell Biol, E-50009 Zaragoza, Spain
来源
JOURNAL OF ANDROLOGY | 2007年 / 28卷 / 05期
关键词
Cryopreservation; pig;
D O I
10.2164/jandrol.107.002725
中图分类号
R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
摘要
The study evaluated the protective effect of seminal plasma (SP) added to freezing extender against cryopreservation injuries to boar spermatozoa. Pooled sperm-rich fractions collected from 9 fertile boars were frozen in 0.5-mL straws after being extended in a conventional freezing extender either alone or supplemented with 5% of SPs (SP1-SP4) collected from the sperm-rich fractions (diluted 1:1, vol/vol, in Beltsville Thawing Solution extender) from 4 boars (1-4) with known sperm cryosurvival (poor, moderate, and good sperm freezers). Cryopreservation injuries were assessed in terms of postthaw sperm motility (assessed by computer-assisted sperm analysis), viability (plasma membrane and acrosome integrity assessed simultaneously by flow cytometry), membrane lipid peroxidation (malonclialdehyde [MDA] production), and the ability of thawed spermatozoa to fertilize in vitro-matured homologous oocytes. The addition of SP from good sperm freezers (SP3 and SP4) improved (P < .01) the motility and viability of thawed spermatozoa without any influence on MDA production. Moreover, SP from good sperm freezers also increased (P < .05) the percentage of penetrated (SP3) and polyspermic oocytes (SP4) with respect to the control. Neither the total amount of SP proteins, protein profiles, nor antioxidant capacity of the different SPs were related to the various cryosurvival/fertilizing capacities of the processed spermatozoa.
引用
收藏
页码:689 / 697
页数:9
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