Immunohistochemical studies on the bovine lactating mammary gland (Bos taurus)

被引：16
作者：
Alkafafy, Mohamed [1 ]
Rashed, Reda [2 ]
Helal, Amr [3 ]
机构：
[1] Menoufia Univ, Sadat City Branch, Dept Cytol & Histol, Fac Vet Med, Sadat City 32897, Minufiya, Egypt
[2] Menoufia Univ, Sadat City Branch, Dept Anat & Embryol, Fac Vet Med, Sadat City 32897, Minufiya, Egypt
[3] Zagazig Univ, Dept Anat & Embryol, Fac Vet Med, Zagazig, Egypt
来源：
ACTA HISTOCHEMICA | 2012年 / 114卷 / 02期
关键词：
Cow; Immunohistochemistry; Mammary gland; Lactation; Myoepithelial cells; ANGIOTENSIN-CONVERTING ENZYME; SMOOTH-MUSCLE CELLS; MYOEPITHELIAL CELLS; ENDOTHELIAL-CELL; EXPRESSION; DIFFERENTIATION; CONNEXINS; PROTEINS; TISSUES; GALACTOSYLTRANSFERASE;
D O I：
10.1016/j.acthis.2011.02.012
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The study aimed to evaluate the validity of immunohistochemistry in the differential labeling of the diverse components of the lactating mammary gland. Paraffin-embedded sections of lactating bovine mammary glands were stained by conventional and histochemical techniques. Primary antibodies against S100, alpha smooth muscle actin (alpha-SMA), connexin-43 (Cx43). cytokeratin-14 (Ck14), galactosyltransferase (GalTase), angiotensin converting enzyme (ACE) and vascular endothelial growth factor (VEGF) were applied on paraffin sections. Strong cytoplasmic and nuclear 5100 immunoreactivity was mainly expressed by alveolar epithelium and to a lesser variable extent by ductal epithelium. The Golgi zone of the epithelial cells expressed strong GalTase immunostaining. Myoepithelial cells displayed a strong immunostaining for a-SMA, Cx43 and Ck14, but not for S100. Vascular endothelium showed a moderate (for VEGF) to strong (for ACE) immunostaining. The presence of VEGF-immunoreactive mast cells within the interstitium may reflect their functional significance in angiogenesis, vascular permeability and migration of mononuclear leukocytes, suggesting their regulatory role in the secretory and immuno-logical functions of the mammary glands. (C) 2011 Elsevier GmbH. All rights reserved.
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收藏
页码：87 / 93
页数：7
相关论文
共 42 条
[1]   Myoepithelial cells: good fences make good neighbors [J].
Adriance, MC ;
Inman, JL ;
Petersen, OW ;
Bissell, MJ .
BREAST CANCER RESEARCH, 2005, 7 (05) :190-197
[2]  
Alkafafy M, 2005, THESIS FACULTY VET M
[3]  
Armesilla AL, 1999, MOL CELL BIOL, V19, P2032
[4]  
Bancroft J.D., 1990, THEORY PRACTICE HIST, V3rd
[5]   The increased level of β1,4-galactosyltransferase required for lactose biosynthesis is achieved in part by translational control [J].
Charron, M ;
Shaper, JH ;
Shaper, NL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14805-14810
[6]   VASCULAR-PERMEABILITY FACTOR - A TUMOR-DERIVED POLYPEPTIDE THAT INDUCES ENDOTHELIAL-CELL AND MONOCYTE PROCOAGULANT ACTIVITY, AND PROMOTES MONOCYTE MIGRATION [J].
CLAUSS, M ;
GERLACH, M ;
GERLACH, H ;
BRETT, J ;
WANG, F ;
FAMILLETTI, PC ;
PAN, YCE ;
OLANDER, JV ;
CONNOLLY, DT ;
STERN, D .
JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (06) :1535-1545
[7]  
Crivellato E, 2004, HISTOL HISTOPATHOL, V19, P259, DOI 10.14670/HH-19.259
[8]   Immunohistochemical distribution of S-100 protein and subunits (S100-α and S100-β) in the swamp-type water buffalo (Bubalus bubalis) testis [J].
Cruzana, MBC ;
Budipitojo, T ;
De Ocampo, G ;
Sasaki, M ;
Kitamura, N ;
Yamada, J .
ANDROLOGIA, 2003, 35 (03) :142-145
[9]  
Danguy A, 1998, ACTA ANAT, V161, P206
[10]   Connexins: a myriad of functions extending beyond assembly of gap junction channels [J].
Dbouk, Hashem A. ;
Mroue, Rana M. ;
El-Sabban, Marwan E. ;
Talhouk, Rabih S. .
CELL COMMUNICATION AND SIGNALING, 2009, 7
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